9AST

Cryo-EM structure of XCR1 signaling complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (L)	Lymphotactin	polymer	133	13905.4	1	UniProt (P47992) Pfam (PF00048)	Homo sapiens (human)	ATAC,C motif chemokine 1,Cytokine SCM-1,Lymphotaxin,SCM-1-alpha,Small-inducible cytokine C1,XC chemokine ligand 1
3	C (R)	Chemokine XC receptor 1,Non structural polyprotein	polymer	532	60383.6	1	UniProt (P46094) UniProt (A0A482LYE4) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 5,Lymphotactin receptor,XC chemokine receptor 1
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7859.2	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (E)	scFv16	polymer	266	28634.8	1		Mus musculus

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

L: 1 - 93 (UniProt: P47992)

PDB	External Database	Details
Cys 21	Val 42	conflict
Cys 59	Val 80	conflict
Gly 94	-	expression tag
Ser 95	-	expression tag
Gly 96	-	expression tag
Ser 97	-	expression tag
Gly 98	-	expression tag
Ser 99	-	expression tag
Gly 100	-	expression tag
Ser 101	-	expression tag
Gly 102	-	expression tag
Ser 103	-	expression tag
Gly 104	-	expression tag
Ser 105	-	expression tag
Gly 106	-	expression tag
Ser 107	-	expression tag
Gly 108	-	expression tag
Ser 109	-	expression tag
Gly 110	-	expression tag
Ser 111	-	expression tag
Gly 112	-	expression tag
Ser 113	-	expression tag
Gly 114	-	expression tag
Ser 115	-	expression tag
Leu 116	-	expression tag
Glu 117	-	expression tag
Val 118	-	expression tag
Leu 119	-	expression tag
Phe 120	-	expression tag
Gln 121	-	expression tag
Gly 122	-	expression tag
Pro 123	-	expression tag
Asp 124	-	expression tag
Tyr 125	-	expression tag
Lys 126	-	expression tag
Asp 127	-	expression tag
Asp 128	-	expression tag
Asp 129	-	expression tag
Asp 130	-	expression tag
Lys 131	-	expression tag
Gly 132	-	expression tag
Ser 133	-	expression tag

R: 2 - 333 (UniProt: P46094)

PDB	External Database	Details
Gly 334	-	linker

R: 335 - 492 (UniProt: A0A482LYE4)

PDB	External Database	Details
Glu 347	Arg 83	conflict
Ala 351	Gly 87	conflict
Leu 367	Phe 103	conflict
Ala 371	Gly 107	conflict
Arg 382	Leu 118	conflict
Ala 387	Gly 123	conflict
Ala 403	Gly 139	conflict
Ala 407	Gly 143	conflict
Glu 411	Lys 147	conflict
Val 412	Ile 148	conflict
Pro 429	His 165	conflict
Leu 443	Ile 179	conflict
Asn 444	Asp 180	conflict
Thr 480	Asn 216	conflict
Met 485	Leu 221	conflict
Ser 493	-	expression tag
Gly 494	-	expression tag
Gly 495	-	expression tag
Ser 496	-	expression tag
Gly 497	-	expression tag
His 498	-	expression tag
His 499	-	expression tag
His 500	-	expression tag
His 501	-	expression tag
His 502	-	expression tag
His 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag
Trp 506	-	expression tag
Ser 507	-	expression tag
His 508	-	expression tag
Pro 509	-	expression tag
Gln 510	-	expression tag
Phe 511	-	expression tag
Glu 512	-	expression tag
Lys 513	-	expression tag
Gly 514	-	expression tag
Gly 515	-	expression tag
Gly 516	-	expression tag
Ser 517	-	expression tag
Gly 518	-	expression tag
Gly 519	-	expression tag
Gly 520	-	expression tag
Ser 521	-	expression tag
Gly 522	-	expression tag
Gly 523	-	expression tag
Ser 524	-	expression tag
Ala 525	-	expression tag
Trp 526	-	expression tag
Ser 527	-	expression tag
His 528	-	expression tag
Pro 529	-	expression tag
Gln 530	-	expression tag
Phe 531	-	expression tag
Glu 532	-	expression tag
Lys 533	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

G: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Gln 17	Glu 17	conflict
Gln 58	Glu 58	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	189941.4
All*	Total formula weight	189941.4

*Water molecules are not included.