9ASH
Cryo-EM structure of the active Lactococcus lactis Csm bound to target in post-cleavage stage
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) | polymer | 759 | 87365.9 | 1 | UniProt (L0CEJ3) | Lactococcus lactis subsp. lactis | Cyclic oligoadenylate synthase |
| 2 | B (B) | CRISPR-associated protein Csm4 | polymer | 297 | 33943.3 | 1 | UniProt (L0CFH1) | Lactococcus lactis subsp. lactis | |
| 3 | C, D, E, F (F, H, G, I) | CRISPR system Cms endoribonuclease Csm3 | polymer | 214 | 23869.2 | 4 | UniProt (L0CEA3) | Lactococcus lactis subsp. lactis | CRISPR type III A-associated RAMP protein Csm3 |
| 4 | G (R) | CRISPR RNA | polymer | 37 | 11946.3 | 1 | Lactococcus lactis subsp. lactis | ||
| 5 | H, I, K (D, E, C) | CRISPR system Cms protein Csm2 | polymer | 150 | 17014.3 | 3 | UniProt (L0CFW2) | Lactococcus lactis subsp. lactis | CRISPR type III A-associated protein Csm2 |
| 6 | J (T) | Target RNA | polymer | 36 | 11558.9 | 1 | Lactococcus lactis subsp. lactis | ||
| 7 | L (J) | CRISPR system Cms protein Csm5 | polymer | 353 | 40621.8 | 1 | UniProt (L0CG31) | Lactococcus lactis subsp. lactis | CRISPR type III A-associated protein Csm5 |
| 8 | M (P) | RNA (5'-D(*(ATP))-R(P*AP*A)-3') | polymer | 3 | 1102.6 | 1 | Lactococcus lactis subsp. lactis | ||
| 9 | N (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 10 | O, P, Q (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 11 | R (A) | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
A: 1 - 757 (UniProt: L0CEJ3)
D, E, C: 12 - 150 (UniProt: L0CFW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 13 | His 13 | conflict |
| Ala 758 | - | expression tag |
| Ala 759 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Gly 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| Ser 9 | - | expression tag |
| Gly 10 | - | expression tag |
| Gly 11 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 333058.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 580.1 | |
| All* | Total formula weight | 333638.5 |
