9ASH
Cryo-EM structure of the active Lactococcus lactis Csm bound to target in post-cleavage stage
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) | polymer | 759 | 87365.9 | 1 | UniProt (L0CEJ3) In PDB | Lactococcus lactis subsp. lactis | Cyclic oligoadenylate synthase |
2 | B | CRISPR-associated protein Csm4 | polymer | 297 | 33943.3 | 1 | UniProt (L0CFH1) In PDB | Lactococcus lactis subsp. lactis | |
3 | F, H, G, I | CRISPR system Cms endoribonuclease Csm3 | polymer | 214 | 23869.2 | 4 | UniProt (L0CEA3) In PDB | Lactococcus lactis subsp. lactis | CRISPR type III A-associated RAMP protein Csm3 |
4 | R | CRISPR RNA | polymer | 37 | 11946.3 | 1 | Lactococcus lactis subsp. lactis | ||
5 | D, E, C | CRISPR system Cms protein Csm2 | polymer | 150 | 17014.3 | 3 | UniProt (L0CFW2) In PDB | Lactococcus lactis subsp. lactis | CRISPR type III A-associated protein Csm2 |
6 | T | Target RNA | polymer | 36 | 11558.9 | 1 | Lactococcus lactis subsp. lactis | ||
7 | J | CRISPR system Cms protein Csm5 | polymer | 353 | 40621.8 | 1 | UniProt (L0CG31) In PDB | Lactococcus lactis subsp. lactis | CRISPR type III A-associated protein Csm5 |
8 | P | RNA (5'-D(*(ATP))-R(P*AP*A)-3') | polymer | 3 | 1102.6 | 1 | Lactococcus lactis subsp. lactis | ||
9 | A | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
10 | A | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
11 | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
A: 1 - 757 (UniProt: L0CEJ3)
D, E, C: 12 - 150 (UniProt: L0CFW2)
PDB | External Database | Details |
---|---|---|
Ala 13 | His 13 | conflict |
Ala 758 | - | expression tag |
Ala 759 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met 1 | - | expression tag |
Gly 2 | - | expression tag |
His 3 | - | expression tag |
His 4 | - | expression tag |
His 5 | - | expression tag |
His 6 | - | expression tag |
His 7 | - | expression tag |
His 8 | - | expression tag |
Ser 9 | - | expression tag |
Gly 10 | - | expression tag |
Gly 11 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 13 |
Total formula weight | 333058.4 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 580.1 | |
All* | Total formula weight | 333638.5 |