9AS2

Global reconstruction of 5-HT2AR bound to DMT in complex with a mini-Gq protein and scFv16 obtained by cryo-electron microscopy (cryoEM)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	5-hydroxytryptamine receptor 2A	polymer	471	52648.9	1	UniProt (P28223) Pfam (PF00001)	Homo sapiens (human)	5-HT-2,5-HT-2A,Serotonin receptor 2A
2	B (B)	G subunit q (Gi2-mini-Gq chimeric)	polymer	246	28084.8	1		Homo sapiens (human)
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
4	D (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (E)	single chain Fab (svFv16)	polymer	267	28668.9	1		Homo sapiens (human)
6	F (A)	2-(1H-indol-3-yl)-N,N-dimethylethan-1-amine	non-polymer		188.3	1	Chemie (A1AFV)

Sequence modifications

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	156681.9
Non-Polymers*	Number of molecules	1
	Total formula weight	188.3
All*	Total formula weight	156870.2

*Water molecules are not included.