8ZWG

cryoEM structure of JR14a bound C3aR-Gi complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-2 adrenergic receptor,C3a anaphylatoxin chemotactic receptor	polymer	538	60236.5	1	UniProt (P07550) UniProt (Q16581) Pfam (PF00001)	Homo sapiens (human)	Beta-2 adrenoreceptor,Beta-2 adrenoceptor,C3AR,C3a-R
2	B (B)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	353	39020.7	1	UniProt (P54311) Pfam (PF25391)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
4	D (E)	scFv16	polymer	267	28737.9	1		synthetic construct
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212) Pfam (PF00631) UniProt (by SIFTS) (A0A1S3WMS4)	Bos taurus (cattle)	G gamma-I
6	F (A)	(2~{S})-5-[bis(azanyl)methylideneamino]-2-[[5-[bis(4-chlorophenyl)methyl]-3-methyl-thiophen-2-yl]carbonylamino]pentanoic acid	non-polymer		533.5	1	Chemie (A1D9A)

Sequence modifications

A: -38 - -9 (UniProt: P07550)

PDB	External Database	Details
Asp -47	-	expression tag
Tyr -46	-	expression tag
Lys -45	-	expression tag
Asp -44	-	expression tag
Asp -43	-	expression tag
Asp -42	-	expression tag
Asp -41	-	expression tag
Val -40	-	expression tag
Asp -39	-	expression tag
Gln -12	Glu 27	engineered mutation
Glu -8	-	linker
Asn -7	-	linker
Leu -6	-	linker
Tyr -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Ala -1	-	linker
Ser 0	-	linker

A: 1 - 482 (UniProt: Q16581)

PDB	External Database	Details
His 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag

B: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	engineered mutation
Ser 326	Ala 326	engineered mutation

C: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -12	-	initiating methionine
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	176003.9
Non-Polymers*	Number of molecules	1
	Total formula weight	533.5
All*	Total formula weight	176537.4

*Water molecules are not included.