8ZUT
Crystal structure of the F99S/M153T/V163A variant of GFP at pH 8.5
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Green fluorescent protein | polymer | 230 | 25844.0 | 1 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria | |
| 2 | B, C (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | D (A) | water | water | 18.0 | 402 | Chemie (HOH) |
Sequence modifications
A: 2 - 231 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Gys 66 | Ser 65 | chromophore |
| Gys 66 | Tyr 66 | chromophore |
| Gys 66 | Gly 67 | chromophore |
| Arg 80 | Gln 80 | conflict |
| Ser 99 | Phe 99 | engineered mutation |
| Thr 153 | Met 153 | engineered mutation |
| Ala 163 | Val 163 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 25844.0 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 25892.6 |
