8ZSS

Cryo-EM structure of the RO5263397-bound hTAAR1-Gs complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	247	28360.0	1	Pfam (PF00503) UniProt (by SIFTS) (P04899) UniProt (by SIFTS) (P63092)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nb35	polymer	139	15271.9	1		Lama glama
5	E (R)	Soluble cytochrome b562,Trace amine-associated receptor 1	polymer	495	56645.2	1	UniProt (P0ABE7) UniProt (Q96RJ0) Pfam (PF07361) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,TaR-1,Trace amine receptor 1
6	F (R)	(4~{S})-4-(3-fluoranyl-2-methyl-phenyl)-1,3-oxazolidin-2-amine	non-polymer		194.2	1	Chemie (A1D8X)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: -113 - -9 (UniProt: P0ABE7)

PDB	External Database	Details
Met -155	-	initiating methionine
Lys -154	-	expression tag
Thr -153	-	expression tag
Ile -152	-	expression tag
Ile -151	-	expression tag
Ala -150	-	expression tag
Leu -149	-	expression tag
Ser -148	-	expression tag
Tyr -147	-	expression tag
Ile -146	-	expression tag
Phe -145	-	expression tag
Cys -144	-	expression tag
Leu -143	-	expression tag
Val -142	-	expression tag
Phe -141	-	expression tag
Ala -140	-	expression tag
Asp -139	-	expression tag
Tyr -138	-	expression tag
Lys -137	-	expression tag
Asp -136	-	expression tag
Asp -135	-	expression tag
Asp -134	-	expression tag
Asp -133	-	expression tag
Lys -132	-	expression tag
His -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
His -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
Leu -121	-	expression tag
Glu -120	-	expression tag
Val -119	-	expression tag
Leu -118	-	expression tag
Phe -117	-	expression tag
Gln -116	-	expression tag
Gly -115	-	expression tag
Pro -114	-	expression tag
Trp -107	Met 29	conflict
Ile -12	His 124	conflict
Leu -8	-	linker
Met -7	-	linker
Gly -6	-	linker
Gln -5	-	linker
Pro -4	-	linker
Gly -3	-	linker
Asn -2	-	linker
Gly -1	-	linker
Ser 0	-	linker
Ala 1	-	linker

R: 2 - 339 (UniProt: Q96RJ0)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	146053.8
Non-Polymers*	Number of molecules	1
	Total formula weight	194.2
All*	Total formula weight	146248.0

*Water molecules are not included.