8ZRK

Cryo-EM structure of GPR119-Gs Complex with small molecule agonist GSK-1292263

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45699.4	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	384	42581.8	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	nanobody35	polymer	157	17057.3	1		synthetic construct
5	R	Glucose-dependent insulinotropic receptor	polymer	369	40762.1	1	UniProt (Q8TDV5) Pfam (PF00001) In PDB	Homo sapiens (human)	G-protein coupled receptor 119
6	R	5-[4-[[6-(4-methylsulfonylphenyl)pyridin-3-yl]oxymethyl]piperidin-1-yl]-3-propan-2-yl-1,2,4-oxadiazole	non-polymer		456.6	1	Chemie (A1D8Y)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	conflict
Ala 226	Gly 226	conflict
Ala 268	Glu 268	conflict
Lys 271	Asn 271	conflict
Asp 274	Lys 274	conflict
Lys 280	Arg 280	conflict
Asp 284	Thr 284	conflict
Thr 285	Ile 285	conflict
Ser 366	Ala 366	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -21	-	initiating methionine
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Val 341	-	expression tag
Ser 342	-	expression tag
Gly 343	-	expression tag
Trp 344	-	expression tag
Arg 345	-	expression tag
Leu 346	-	expression tag
Phe 347	-	expression tag
Lys 348	-	expression tag
Lys 349	-	expression tag
Ile 350	-	expression tag
Ser 351	-	expression tag
Val 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Trp 355	-	expression tag
Arg 356	-	expression tag
Leu 357	-	expression tag
Phe 358	-	expression tag
Lys 359	-	expression tag
Lys 360	-	expression tag
Ile 361	-	expression tag
Ser 362	-	expression tag

R: 1 - 335 (UniProt: Q8TDV5)

PDB	External Database	Details
Met -33	-	initiating methionine
Lys -32	-	expression tag
Thr -31	-	expression tag
Ile -30	-	expression tag
Ile -29	-	expression tag
Ala -28	-	expression tag
Leu -27	-	expression tag
Ser -26	-	expression tag
Tyr -25	-	expression tag
Ile -24	-	expression tag
Phe -23	-	expression tag
Cys -22	-	expression tag
Leu -21	-	expression tag
Val -20	-	expression tag
Phe -19	-	expression tag
Ala -18	-	expression tag
Asp -17	-	expression tag
Tyr -16	-	expression tag
Lys -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Lys -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	153961.8
Non-Polymers*	Number of molecules	1
	Total formula weight	456.6
All*	Total formula weight	154418.3

*Water molecules are not included.