8ZP5
Cryo-EM structure of origin recognition complex (Orc5 basic patch mutations) with ARS1 DNA bound
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Origin recognition complex subunit 2 | polymer | 620 | 71342.2 | 1 | UniProt (P32833) Pfam (PF04084) | Saccharomyces cerevisiae S288C | Origin recognition complex 71 kDa subunit |
| 2 | B (E) | Origin recognition complex subunit 5 | polymer | 479 | 55116.8 | 1 | UniProt (P50874) Pfam (PF21639) Pfam (PF14630) Pfam (PF13191) | Saccharomyces cerevisiae S288C | Origin recognition complex 53 kDa subunit |
| 3 | C (F) | Origin recognition complex subunit 6 | polymer | 435 | 50369.5 | 1 | UniProt (P38826) Pfam (PF05460) | Saccharomyces cerevisiae S288C | ACS-associated protein 1,Origin recognition complex 50 kDa subunit |
| 4 | D (G) | DNA (35-MER) | polymer | 77 | 23708.3 | 1 | Saccharomyces cerevisiae | ||
| 5 | E (H) | DNA (34-MER) | polymer | 77 | 23766.3 | 1 | Saccharomyces cerevisiae | ||
| 6 | F (A) | Origin recognition complex subunit 1 | polymer | 914 | 104546.2 | 1 | UniProt (P54784) Pfam (PF01426) Pfam (PF00004) Pfam (PF17872) Pfam (PF21312) | Saccharomyces cerevisiae S288C | Origin recognition complex 120 kDa subunit |
| 7 | G (C) | Origin recognition complex subunit 3 | polymer | 616 | 72161.8 | 1 | UniProt (P54790) Pfam (PF07034) Pfam (PF18137) | Saccharomyces cerevisiae S288C | Origin recognition complex 62 kDa subunit |
| 8 | H (D) | Origin recognition complex subunit 4 | polymer | 529 | 60772.2 | 1 | UniProt (P54791) Pfam (PF14629) | Saccharomyces cerevisiae S288C | Origin recognition complex 56 kDa subunit |
| 9 | I, K, M (E, A, D) | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 3 | Chemie (AGS) | |||
| 10 | J, L, N (E, A, D) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) |
Sequence modifications
E: 1 - 479 (UniProt: P50874)
| PDB | External Database | Details |
|---|---|---|
| Ala 360 | Arg 360 | engineered mutation |
| Ala 366 | Arg 366 | engineered mutation |
| Ala 367 | Lys 367 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 461783.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1642.7 | |
| All* | Total formula weight | 463425.8 |
