8ZP5
Cryo-EM structure of origin recognition complex (Orc5 basic patch mutations) with ARS1 DNA bound
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.98 |
| Number of Particle | 1387713 |
| Software | Gautomatch,cryoSPARC,EPU,Gctf,UCSF Chimera,PHENIX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
