8ZNC
Cryo-EM structure of W89F mutated Glutamate dehydrogenase from Thermococcus profundus incorporating NADPH, AKG in the steady stage of reaction
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Glutamate dehydrogenase | polymer | 416 | 46360.0 | 1 | UniProt (O74024) Pfam (PF02812) Pfam (PF00208) | Thermococcus profundus | GDH |
| 2 | B (A) | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 1 | Chemie (NDP) | |||
| 3 | C (A) | 2-OXOGLUTARIC ACID | non-polymer | 146.1 | 1 | Chemie (AKG) | |||
| 4 | D (A) | AMMONIUM ION | non-polymer | 18.0 | 1 | Chemie (NH4) | |||
| 5 | E (A) | water | water | 18.0 | 24 | Chemie (HOH) |
Sequence modifications
A: 4 - 419 (UniProt: O74024)
| PDB | External Database | Details |
|---|---|---|
| Phe 89 | Trp 89 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 46360.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 909.6 | |
| All* | Total formula weight | 47269.6 |
