8ZK7

Crystal structure of the Decarboxylase KDC4427 mutant E468L from Enterobacter sp. CGMCC 5087

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Indolepyruvate decarboxylase	polymer	566	61941.4	2	UniProt (A0A2U3EYQ2) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775)	Enterobacter sp. CGMCC 5087	KDC4427
2	C, E (A, B)	THIAMINE DIPHOSPHATE	non-polymer		425.3	2	Chemie (TPP)
3	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	G, H (A, B)	water	water		18.0	330	Chemie (HOH)

Sequence modifications

A, B: 1 - 552 (UniProt: A0A2U3EYQ2)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Leu 468	Glu 468	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	123882.7
Non-Polymers*	Number of molecules	4
	Total formula weight	899.2
All*	Total formula weight	124782.0

*Water molecules are not included.