8ZK0

Cryo-EM structure of the full length pAg-bound BTN2A1-BTN3A1-BTN3A2 in complex with Vgamma9-Vdelta2 TCR (G115 genotype)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Butyrophilin subfamily 2 member A1	polymer	534	60684.5	2	UniProt (Q7KYR7) Pfam (PF07686) Pfam (PF22705) Pfam (PF13765) Pfam (PF00622)	Homo sapiens (human)
2	C (D)	T cell receptor delta variable 2, T cell receptor delta constant 1	polymer	239	26489.0	1		Homo sapiens
3	D (G)	T cell receptor gamma variable 9, T cell receptor gamma constant 1	polymer	252	28328.3	1		Homo sapiens
4	E (E)	Butyrophilin subfamily 3 member A2	polymer	340	37175.1	1	UniProt (P78410) Pfam (PF07686) Pfam (PF22705)	Homo sapiens (human)
5	F (F)	Butyrophilin subfamily 3 member A1	polymer	539	60102.3	1	UniProt (O00481) Pfam (PF00622) Pfam (PF07686) Pfam (PF22705) Pfam (PF13765)	Homo sapiens (human)
6	G (F)	(2E)-4-hydroxy-3-methylbut-2-en-1-yl trihydrogen diphosphate	non-polymer		262.1	1	Chemie (H6P)

Sequence modifications

A, B: 29 - 527 (UniProt: Q7KYR7)

PDB	External Database	Details
Met 7	-	initiating methionine
Asp 8	-	expression tag
Met 9	-	expression tag
Arg 10	-	expression tag
Val 11	-	expression tag
Pro 12	-	expression tag
Ala 13	-	expression tag
Gln 14	-	expression tag
Leu 15	-	expression tag
Leu 16	-	expression tag
Gly 17	-	expression tag
Leu 18	-	expression tag
Leu 19	-	expression tag
Leu 20	-	expression tag
Leu 21	-	expression tag
Trp 22	-	expression tag
Leu 23	-	expression tag
Ser 24	-	expression tag
Gly 25	-	expression tag
Ala 26	-	expression tag
Arg 27	-	expression tag
Cys 28	-	expression tag
Gly 528	-	expression tag
Ser 529	-	expression tag
Ser 530	-	expression tag
Gly 531	-	expression tag
Met 532	-	expression tag
Asp 533	-	expression tag
Tyr 534	-	expression tag
Lys 535	-	expression tag
Asp 536	-	expression tag
Asp 537	-	expression tag
Asp 538	-	expression tag
Asp 539	-	expression tag
Lys 540	-	expression tag

E: 30 - 334 (UniProt: P78410)

PDB	External Database	Details
Met 8	-	initiating methionine
Asp 9	-	expression tag
Met 10	-	expression tag
Arg 11	-	expression tag
Val 12	-	expression tag
Pro 13	-	expression tag
Ala 14	-	expression tag
Gln 15	-	expression tag
Leu 16	-	expression tag
Leu 17	-	expression tag
Gly 18	-	expression tag
Leu 19	-	expression tag
Leu 20	-	expression tag
Leu 21	-	expression tag
Leu 22	-	expression tag
Trp 23	-	expression tag
Leu 24	-	expression tag
Ser 25	-	expression tag
Gly 26	-	expression tag
Ala 27	-	expression tag
Arg 28	-	expression tag
Cys 29	-	expression tag
Gly 335	-	expression tag
Ser 336	-	expression tag
Ser 337	-	expression tag
Gly 338	-	expression tag
Met 339	-	expression tag
Asp 340	-	expression tag
Tyr 341	-	expression tag
Lys 342	-	expression tag
Asp 343	-	expression tag
Asp 344	-	expression tag
Asp 345	-	expression tag
Asp 346	-	expression tag
Lys 347	-	expression tag

F: 30 - 513 (UniProt: O00481)

PDB	External Database	Details
Met 8	-	initiating methionine
Asp 9	-	expression tag
Met 10	-	expression tag
Arg 11	-	expression tag
Val 12	-	expression tag
Pro 13	-	expression tag
Ala 14	-	expression tag
Gln 15	-	expression tag
Leu 16	-	expression tag
Leu 17	-	expression tag
Gly 18	-	expression tag
Leu 19	-	expression tag
Leu 20	-	expression tag
Leu 21	-	expression tag
Leu 22	-	expression tag
Trp 23	-	expression tag
Leu 24	-	expression tag
Ser 25	-	expression tag
Gly 26	-	expression tag
Ala 27	-	expression tag
Arg 28	-	expression tag
Cys 29	-	expression tag
Gly 514	-	expression tag
Ser 515	-	expression tag
Ser 516	-	expression tag
Gly 517	-	expression tag
Ala 518	-	expression tag
Trp 519	-	expression tag
Ser 520	-	expression tag
His 521	-	expression tag
Pro 522	-	expression tag
Gln 523	-	expression tag
Phe 524	-	expression tag
Glu 525	-	expression tag
Lys 526	-	expression tag
Gly 527	-	expression tag
Gly 528	-	expression tag
Gly 529	-	expression tag
Ser 530	-	expression tag
Gly 531	-	expression tag
Gly 532	-	expression tag
Gly 533	-	expression tag
Ser 534	-	expression tag
Gly 535	-	expression tag
Gly 536	-	expression tag
Ser 537	-	expression tag
Ala 538	-	expression tag
Trp 539	-	expression tag
Ser 540	-	expression tag
His 541	-	expression tag
Pro 542	-	expression tag
Gln 543	-	expression tag
Phe 544	-	expression tag
Glu 545	-	expression tag
Lys 546	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	273463.5
Non-Polymers*	Number of molecules	1
	Total formula weight	262.1
All*	Total formula weight	273725.6

*Water molecules are not included.