8ZJ2

Cryo-EM structure of the RhoG/DOCK5/ELMO1/Rac1 complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, F	Dedicator of cytokinesis protein 5	polymer	1648	191492.1	2	UniProt (Q9H7D0) Pfam (PF00018) Pfam (PF16172) Pfam (PF14429) Pfam (PF06920) Pfam (PF20422) Pfam (PF20421) In PDB	Homo sapiens (human)
2	C, G	Ras-related C3 botulinum toxin substrate 1	polymer	184	20244.3	2	UniProt (P63000) Pfam (PF00071) In PDB	Homo sapiens (human)	Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1
3	E, A	Engulfment and cell motility protein 1	polymer	733	84337.7	2	UniProt (Q92556) Pfam (PF11841) Pfam (PF04727) Pfam (PF16457) In PDB	Homo sapiens (human)	Protein ced-12 homolog
4	D	Rho-related GTP-binding protein RhoG	polymer	203	22362.4	1	UniProt (P84095) Pfam (PF00071) In PDB	Homo sapiens (human)
5	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	D	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)

Sequence modifications

B, F: 1 - 1642 (UniProt: Q9H7D0)

PDB	External Database	Details
Gly -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Arg 1285	Lys 1285	variant

C, G: 1 - 177 (UniProt: P63000)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Ala 15	Gly 15	engineered mutation

E, A: 1 - 727 (UniProt: Q92556)

PDB	External Database	Details
Gly -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

D: 1 - 184 (UniProt: P84095)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Leu 61	Gln 61	engineered mutation
Ser 185	-	expression tag
Gly 186	-	expression tag
Pro 187	-	expression tag
Ser 188	-	expression tag
Ser 189	-	expression tag
Gly 190	-	expression tag
Glu 191	-	expression tag
Asn 192	-	expression tag
Leu 193	-	expression tag
Tyr 194	-	expression tag
Phe 195	-	expression tag
Gln 196	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	614510.6
Non-Polymers*	Number of molecules	2
	Total formula weight	547.5
All*	Total formula weight	615058.0

*Water molecules are not included.