8ZH8

Human GPR103 -Gq complex bound to QRFP26

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Pyroglutamylated RF-amide peptide receptor	polymer	402	45652.0	1	UniProt (Q96P65) Pfam (PF00001)	Homo sapiens (human)	AQ27,G-protein coupled receptor 103,Orexigenic neuropeptide QRFP receptor,SP9155
2	B (N)	nanobody Nb35	polymer	137	15015.7	1		Lama glama
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7547.7	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (cattle)	G gamma-I
5	E (A)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2,Guanine nucleotide-binding protein G(i) subunit alpha-2,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas	polymer	326	36573.5	1	UniProt (P59768) UniProt (P04899) UniProt (Q5JWF2) Pfam (PF00631) Pfam (PF00503)	Homo sapiens (human)	G gamma-I,Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein,Extra large alphas protein,XLalphas
6	F (S)	scFv16	polymer	260	27720.8	1		Mus musculus
7	G (Q)	QRF-amide	polymer	27	2835.2	1	UniProt (P83859)	Homo sapiens	Neuropeptide RF-amide,Pyroglutamylated arginine-phenylalanine-amide peptide

Sequence modifications

R: 2 - 366 (UniProt: Q96P65)

PDB	External Database	Details
Met -22	-	initiating methionine
Lys -21	-	expression tag
Thr -20	-	expression tag
Ile -19	-	expression tag
Ile -18	-	expression tag
Ala -17	-	expression tag
Leu -16	-	expression tag
Ser -15	-	expression tag
Tyr -14	-	expression tag
Ile -13	-	expression tag
Phe -12	-	expression tag
Cys -11	-	expression tag
Leu -10	-	expression tag
Val -9	-	expression tag
Phe -8	-	expression tag
Ala -7	-	expression tag
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag
Ser 367	-	expression tag
Gly 368	-	expression tag
Gly 369	-	expression tag
Gly 370	-	expression tag
Gly 371	-	expression tag
Ser 372	-	expression tag
Gly 373	-	expression tag
Gly 374	-	expression tag
Ser 375	-	expression tag
Ser 376	-	expression tag
Ser 377	-	expression tag
Gly 378	-	expression tag
Gly 379	-	expression tag

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

G: 1 - 67 (UniProt: P63212)

PDB	External Database	Details
Ser 68	-	expression tag

A: -79 - -9 (UniProt: P59768)

PDB	External Database	Details
Gly -8	-	linker
Ser -7	-	linker
Ala -6	-	linker
Gly -5	-	linker
Ser -4	-	linker
Ala -3	-	linker
Gly -2	-	linker
Ser -1	-	linker
Ala 0	-	linker

A: 1 - 57 (UniProt: P04899)

PDB	External Database	Details
Ser 3	Cys 3	engineered mutation
Arg 31	Ala 31	engineered mutation
Thr 33	Glu 33	engineered mutation
Leu 34	Val 34	engineered mutation
Arg 35	Lys 35	engineered mutation
Asp 42	Gly 42	engineered mutation
Asn 43	Glu 43	engineered mutation
Arg 54	Lys 54	engineered mutation
Leu 56	Ile 56	engineered mutation
Gly 58	-	linker
Gly 59	-	linker
Ser 60	-	linker
Gly 61	-	linker
Gly 62	-	linker
Ser 63	-	linker
Gly 64	-	linker
Gly 65	-	linker

A: 66 - 246 (UniProt: Q5JWF2)

PDB	External Database	Details
Asp 111	Ala 892	engineered mutation
Asp 114	Ser 895	engineered mutation
-	Asn 897	deletion
-	Met 898	deletion
-	Val 899	deletion
-	Ile 900	deletion
-	Arg 901	deletion
-	Glu 902	deletion
-	Asp 903	deletion
-	Asn 904	deletion
-	Gln 905	deletion
-	Thr 906	deletion
Asp 124	Leu 915	engineered mutation
Lys 195	Asp 986	engineered mutation
Val 198	Leu 989	engineered mutation
Asp 199	Arg 990	engineered mutation
Ile 210	Tyr 1001	engineered mutation
Ala 224	Ile 1015	engineered mutation
Ile 227	Val 1018	engineered mutation
Lys 232	Arg 1023	engineered mutation
Leu 236	Gln 1027	engineered mutation
Gln 237	Arg 1028	engineered mutation
Asn 239	His 1030	engineered mutation
Glu 242	Gln 1033	engineered mutation
Asn 244	Glu 1035	engineered mutation
Val 246	Leu 1037	engineered mutation

Q: 1 - 26 (UniProt: P83859)

PDB	External Database	Details
Nh2 27	-	amidation

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	174089.3
All*	Total formula weight	174089.3

*Water molecules are not included.