8ZAR

EmrAB-TolC MFS-type tripartite multidrug efflux pump FA

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Multidrug export protein EmrA	polymer	401	43978.0	6	UniProt (P27303) Pfam (PF00529) Pfam (PF16576)	Escherichia coli K-12
2	G (J)	Multidrug export protein EmrB	polymer	614	67336.3	1	UniProt (P0AEJ0) Pfam (PF07690)	Escherichia coli K-12
3	H, I, J (G, H, I)	Outer membrane protein TolC	polymer	501	54794.3	3	UniProt (P02930) Pfam (PF02321)	Escherichia coli K-12	Multidrug efflux pump subunit TolC,Outer membrane factor TolC

Sequence modifications

A, B, C, D, E, F: 1 - 390 (UniProt: P27303)

PDB	External Database	Details
Gly 391	-	expression tag
Gly 392	-	expression tag
Ser 393	-	expression tag
Asp 394	-	expression tag
Tyr 395	-	expression tag
Lys 396	-	expression tag
Asp 397	-	expression tag
Asp 398	-	expression tag
Asp 399	-	expression tag
Asp 400	-	expression tag
Lys 401	-	expression tag

J: 10 - 503 (UniProt: P0AEJ0)

PDB	External Database	Details
Met -110	-	initiating methionine
Gly -109	-	expression tag
Met -108	-	expression tag
Thr -107	-	expression tag
Pro -106	-	expression tag
Leu -105	-	expression tag
Ala -104	-	expression tag
Asp -103	-	expression tag
Leu -102	-	expression tag
Glu -101	-	expression tag
Asp -100	-	expression tag
Asn -99	-	expression tag
Trp -98	-	expression tag
Glu -97	-	expression tag
Thr -96	-	expression tag
Leu -95	-	expression tag
Asn -94	-	expression tag
Asp -93	-	expression tag
Asn -92	-	expression tag
Leu -91	-	expression tag
Lys -90	-	expression tag
Val -89	-	expression tag
Ile -88	-	expression tag
Glu -87	-	expression tag
Lys -86	-	expression tag
Ala -85	-	expression tag
Asp -84	-	expression tag
Asn -83	-	expression tag
Ala -82	-	expression tag
Ala -81	-	expression tag
Gln -80	-	expression tag
Val -79	-	expression tag
Lys -78	-	expression tag
Asp -77	-	expression tag
Ala -76	-	expression tag
Leu -75	-	expression tag
Thr -74	-	expression tag
Lys -73	-	expression tag
Met -72	-	expression tag
Arg -71	-	expression tag
Ala -70	-	expression tag
Ala -69	-	expression tag
Ala -68	-	expression tag
Leu -67	-	expression tag
Asp -66	-	expression tag
Ala -65	-	expression tag
Gln -64	-	expression tag
Lys -63	-	expression tag
Ala -62	-	expression tag
Thr -61	-	expression tag
Pro -60	-	expression tag
Pro -59	-	expression tag
Lys -58	-	expression tag
Leu -57	-	expression tag
Glu -56	-	expression tag
Asp -55	-	expression tag
Lys -54	-	expression tag
Ser -53	-	expression tag
Pro -52	-	expression tag
Asp -51	-	expression tag
Ser -50	-	expression tag
Pro -49	-	expression tag
Glu -48	-	expression tag
Met -47	-	expression tag
Lys -46	-	expression tag
Asp -45	-	expression tag
Phe -44	-	expression tag
Arg -43	-	expression tag
His -42	-	expression tag
Gly -41	-	expression tag
Phe -40	-	expression tag
Asp -39	-	expression tag
Ile -38	-	expression tag
Leu -37	-	expression tag
Val -36	-	expression tag
Gly -35	-	expression tag
Gln -34	-	expression tag
Ile -33	-	expression tag
Asp -32	-	expression tag
Asp -31	-	expression tag
Ala -30	-	expression tag
Leu -29	-	expression tag
Lys -28	-	expression tag
Leu -27	-	expression tag
Ala -26	-	expression tag
Asn -25	-	expression tag
Glu -24	-	expression tag
Gly -23	-	expression tag
Lys -22	-	expression tag
Val -21	-	expression tag
Lys -20	-	expression tag
Glu -19	-	expression tag
Ala -18	-	expression tag
Gln -17	-	expression tag
Ala -16	-	expression tag
Ala -15	-	expression tag
Ala -14	-	expression tag
Glu -13	-	expression tag
Gln -12	-	expression tag
Leu -11	-	expression tag
Lys -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Arg -7	-	expression tag
Asn -6	-	expression tag
Ala -5	-	expression tag
Tyr -4	-	expression tag
Ile -3	-	expression tag
Gln -2	-	expression tag
Lys -1	-	expression tag
Tyr 0	-	expression tag
Leu 1	-	expression tag
Glu 2	-	expression tag
Arg 3	-	expression tag
Ala 4	-	expression tag
Arg 5	-	expression tag
Ser 6	-	expression tag
Thr 7	-	expression tag
Leu 8	-	expression tag
Met 9	-	expression tag

G, H, I: -21 - 471 (UniProt: P02930)

PDB	External Database	Details
Leu 169	Val 191	engineered mutation
Asp 472	-	expression tag
Tyr 473	-	expression tag
Lys 474	-	expression tag
Asp 475	-	expression tag
Asp 476	-	expression tag
Asp 477	-	expression tag
Asp 478	-	expression tag
Lys 479	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	495587.2
All*	Total formula weight	495587.2

*Water molecules are not included.