8Z9P
Cryo-EM structure of human GPR4-Gi complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P54311) Pfam (PF25391) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 2 | B (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (domestic cattle) | G gamma-I |
| 3 | C (R) | G-protein coupled receptor 4 | polymer | 303 | 34657.5 | 1 | UniProt (P46093) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 6C.l,GPR6C.l |
| 4 | D (C) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 5 | E (S) | single Fab chain (svFv16) | polymer | 250 | 26610.6 | 1 | synthetic construct | ||
| 6 | F, G (R) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) |
Sequence modifications
B: 2 - 340 (UniProt: P54311)
C: 1 - 354 (UniProt: P63096)
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 147327.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 773.3 | |
| All* | Total formula weight | 148100.9 |
