8Z5F

Crystal structure of beta-ketoacyl-ACP synthase FabF K336A in complex with decanoyl-ACP from Helicobacter pylori

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	3-oxoacyl-[acyl-carrier-protein] synthase 2	polymer	412	43497.6	2	UniProt (A0A438WLJ1) UniProt (by SIFTS) (V6LC02)	Helicobacter pylori (Campylobacter pylori)
2	C, D (C, D)	Acyl carrier protein	polymer	86	9363.5	2	UniProt (Q5EDC8) UniProt (by SIFTS) (B6JLE2)	Helicobacter pylori (Campylobacter pylori)	ACP
3	E, F (A, B)	DECANOIC ACID	non-polymer		172.3	2	Chemie (DKA)	Helicobacter pylori
4	G, H (C, D)	N~3~-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-N-(2-sulfanylethyl)-beta-alaninamide	non-polymer		358.3	2	Chemie (PN7)	Helicobacter pylori
5	I, J, K, L (A, B, C, D)	water	water		18.0	310	Chemie (HOH)

Sequence modifications

A, B: 1 - 412 (UniProt: A0A438WLJ1)

PDB	External Database	Details
Ala 336	Lys 336	engineered mutation

C, D: 1 - 78 (UniProt: Q5EDC8)

PDB	External Database	Details
Gly -7	-	expression tag
Thr -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Met -3	-	expression tag
Gly -2	-	expression tag
Tyr -1	-	expression tag
Leu 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	105722.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1061.2
All*	Total formula weight	106783.5

*Water molecules are not included.