8Z5F
Crystal structure of beta-ketoacyl-ACP synthase FabF K336A in complex with decanoyl-ACP from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9875 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.312, 153.586, 72.442 |
| Unit cell angles | 90.00, 113.28, 90.00 |
Refinement procedure
| Resolution | 33.270 - 1.950 |
| R-factor | 0.4238 |
| Rwork | 0.272 |
| R-free | 0.27760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.162 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.270 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.144 | 0.572 |
| Rmeas | 0.158 | 0.666 |
| Rpim | 0.063 | 0.330 |
| Total number of observations | 471141 | 15640 |
| Number of reflections | 80031 | 4390 |
| <I/σ(I)> | 5.7 | 1.5 |
| Completeness [%] | 95.9 | |
| Redundancy | 5.9 | 3.6 |
| CC(1/2) | 0.994 | 0.786 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Tris, pH6.5, 15% w/v PEG 4000 and 20% w/v glycerin |
