8Z2Q

Crystal structure of trehalose synthase mutant N253Q from Deinococcus radiodurans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	maltose alpha-D-glucosyltransferase	polymer	571	65001.9	8	UniProt (I3NX86) Pfam (PF00128) Pfam (PF16657)	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)	Maltose alpha-D-glucosyltransferase
2	BA, EA, I, L, P... (G, H, A, B, C...)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
3	CA, FA, J, M, Q... (G, H, A, B, C...)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
4	AA, DA, GA, K, N... (F, G, H, A, B...)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	9	Chemie (TRS)
5	HA, IA, JA, KA, LA... (A, B, C, D, E...)	water	water		18.0	794	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 2 - 552 (UniProt: I3NX86)

PDB	External Database	Details
Met -1	-	initiating methionine
Val 0	-	expression tag
Pro 1	-	expression tag
Trp 97	Arg 97	engineered mutation
Gln 253	Asn 253	engineered mutation
Ile 313	Thr 313	engineered mutation
Val 380	Ile 380	engineered mutation
Ser 553	-	expression tag
Arg 554	-	expression tag
Val 555	-	expression tag
Asp 556	-	expression tag
Lys 557	-	expression tag
Leu 558	-	expression tag
Ala 559	-	expression tag
Ala 560	-	expression tag
Ala 561	-	expression tag
Leu 562	-	expression tag
Glu 563	-	expression tag
His 564	-	expression tag
His 565	-	expression tag
His 566	-	expression tag
His 567	-	expression tag
His 568	-	expression tag
His 569	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	520014.9
Non-Polymers*	Number of molecules	25
	Total formula weight	1614.4
All*	Total formula weight	521629.3

*Water molecules are not included.