8Z2Q
Crystal structure of trehalose synthase mutant N253Q from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.989, 198.602, 134.117 |
| Unit cell angles | 90.00, 91.01, 90.00 |
Refinement procedure
| Resolution | 20.080 - 2.990 |
| R-factor | 0.1586 |
| Rwork | 0.154 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.035 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.080 | 3.070 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.270 | 0.166 |
| Number of reflections | 99276 | 94332 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris-HCl pH 8.5, 8% (w/v) polyethylene glycol (PEG) 8000 |
