8YW4
Cryo-EM structure of the retatrutide-bound human GIPR-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Mini-Gs | polymer | 361 | 41879.5 | 1 | Pfam (PF00503) UniProt (by SIFTS) (P63092) | Homo sapiens | |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 341 | 37429.9 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (N) | Nanobody-35 | polymer | 140 | 15343.0 | 1 | synthetic construct | ||
| 5 | E (R) | Gastric inhibitory polypeptide receptor | polymer | 573 | 65186.9 | 1 | UniProt (P48546) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (human) | GIP-R,Glucose-dependent insulinotropic polypeptide receptor |
| 6 | F (P) | retatrutide | polymer | 27 | 3114.5 | 1 | Homo sapiens |
Sequence modifications
B: 3 - 340 (UniProt: P62873)
R: 22 - 421 (UniProt: P48546)
R: 422 - 594 (PDB: 8YW4)
| PDB | External Database | Details |
|---|---|---|
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Thr 95 | Ala 95 | conflict |
| Glu 99 | Val 99 | conflict |
| Tyr 101 | Arg 101 | conflict |
| Arg 108 | Gln 108 | conflict |
| Glu 110 | Gly 110 | conflict |
| Arg 111 | Leu 111 | conflict |
| Tyr 112 | Trp 112 | conflict |
| Arg 121 | Pro 121 | conflict |
| Cys 123 | Lys 123 | conflict |
| Gln 124 | Asn 124 | conflict |
| Phe 345 | Thr 345 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 170814.9 | |
| All* | Total formula weight | 170814.9 |
