8YUT

Cryo-EM structure of the amthamine-bound H2R-Gs complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	378	43883.8	1		Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	356	39141.8	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	Nanobody-35	polymer	128	13885.4	1		synthetic construct
5	R	Histamine H2 receptor	polymer	411	46456.0	1	UniProt (P25021) Pfam (PF00001) In PDB	Homo sapiens (human)	H2R,HH2R,Gastric receptor I
6	R	5-(2-azanylethyl)-4-methyl-1,3-thiazol-2-amine	non-polymer		157.2	1	Chemie (A1D67)
7	R	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 2 - 359 (UniProt: P25021)

PDB	External Database	Details
Met -32	-	initiating methionine
Lys -31	-	expression tag
Thr -30	-	expression tag
Ile -29	-	expression tag
Ile -28	-	expression tag
Ala -27	-	expression tag
Leu -26	-	expression tag
Ser -25	-	expression tag
Tyr -24	-	expression tag
Ile -23	-	expression tag
Phe -22	-	expression tag
Cys -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Phe -18	-	expression tag
Ala -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Glu -8	-	expression tag
Phe -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Pro 1	-	expression tag
Leu 360	-	expression tag
Glu 361	-	expression tag
Glu 362	-	expression tag
Asn 363	-	expression tag
Leu 364	-	expression tag
Tyr 365	-	expression tag
Phe 366	-	expression tag
Gln 367	-	expression tag
Gly 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	151228.2
Non-Polymers*	Number of molecules	2
	Total formula weight	543.9
All*	Total formula weight	151772.1

*Water molecules are not included.