8YUT
Cryo-EM structure of the amthamine-bound H2R-Gs complex
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 378 | 43883.8 | 1 | Pfam (PF00503) UniProt (by SIFTS) (P63092) UniProt (by SIFTS) (P63096) | Homo sapiens (human) | |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39141.8 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (N) | Nanobody-35 | polymer | 128 | 13885.4 | 1 | synthetic construct | ||
| 5 | E (R) | Histamine H2 receptor | polymer | 411 | 46456.0 | 1 | UniProt (P25021) Pfam (PF00001) | Homo sapiens (human) | H2R,HH2R,Gastric receptor I |
| 6 | F (R) | 5-(2-azanylethyl)-4-methyl-1,3-thiazol-2-amine | non-polymer | 157.2 | 1 | Chemie (A1D67) | |||
| 7 | G (R) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: 2 - 359 (UniProt: P25021)
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -32 | - | initiating methionine |
| Lys -31 | - | expression tag |
| Thr -30 | - | expression tag |
| Ile -29 | - | expression tag |
| Ile -28 | - | expression tag |
| Ala -27 | - | expression tag |
| Leu -26 | - | expression tag |
| Ser -25 | - | expression tag |
| Tyr -24 | - | expression tag |
| Ile -23 | - | expression tag |
| Phe -22 | - | expression tag |
| Cys -21 | - | expression tag |
| Leu -20 | - | expression tag |
| Val -19 | - | expression tag |
| Phe -18 | - | expression tag |
| Ala -17 | - | expression tag |
| Asp -16 | - | expression tag |
| Tyr -15 | - | expression tag |
| Lys -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Lys -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Phe -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Val -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
| Leu 360 | - | expression tag |
| Glu 361 | - | expression tag |
| Glu 362 | - | expression tag |
| Asn 363 | - | expression tag |
| Leu 364 | - | expression tag |
| Tyr 365 | - | expression tag |
| Phe 366 | - | expression tag |
| Gln 367 | - | expression tag |
| Gly 368 | - | expression tag |
| His 369 | - | expression tag |
| His 370 | - | expression tag |
| His 371 | - | expression tag |
| His 372 | - | expression tag |
| His 373 | - | expression tag |
| His 374 | - | expression tag |
| His 375 | - | expression tag |
| His 376 | - | expression tag |
| His 377 | - | expression tag |
| His 378 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 151228.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 543.9 | |
| All* | Total formula weight | 151772.1 |
