8YUT
Cryo-EM structure of the amthamine-bound H2R-Gs complex
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 378 | 43883.8 | 1 | Homo sapiens (human) | ||
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39141.8 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
4 | N | Nanobody-35 | polymer | 128 | 13885.4 | 1 | synthetic construct | ||
5 | R | Histamine H2 receptor | polymer | 411 | 46456.0 | 1 | UniProt (P25021) Pfam (PF00001) In PDB | Homo sapiens (human) | H2R,HH2R,Gastric receptor I |
6 | R | 5-(2-azanylethyl)-4-methyl-1,3-thiazol-2-amine | non-polymer | 157.2 | 1 | Chemie (A1D67) | |||
7 | R | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: 2 - 359 (UniProt: P25021)
PDB | External Database | Details |
---|---|---|
Met -15 | - | initiating methionine |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Leu -10 | - | expression tag |
Glu -9 | - | expression tag |
Val -8 | - | expression tag |
Leu -7 | - | expression tag |
Phe -6 | - | expression tag |
Gln -5 | - | expression tag |
Gly -4 | - | expression tag |
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -32 | - | initiating methionine |
Lys -31 | - | expression tag |
Thr -30 | - | expression tag |
Ile -29 | - | expression tag |
Ile -28 | - | expression tag |
Ala -27 | - | expression tag |
Leu -26 | - | expression tag |
Ser -25 | - | expression tag |
Tyr -24 | - | expression tag |
Ile -23 | - | expression tag |
Phe -22 | - | expression tag |
Cys -21 | - | expression tag |
Leu -20 | - | expression tag |
Val -19 | - | expression tag |
Phe -18 | - | expression tag |
Ala -17 | - | expression tag |
Asp -16 | - | expression tag |
Tyr -15 | - | expression tag |
Lys -14 | - | expression tag |
Asp -13 | - | expression tag |
Asp -12 | - | expression tag |
Asp -11 | - | expression tag |
Asp -10 | - | expression tag |
Lys -9 | - | expression tag |
Glu -8 | - | expression tag |
Phe -7 | - | expression tag |
Leu -6 | - | expression tag |
Glu -5 | - | expression tag |
Val -4 | - | expression tag |
Leu -3 | - | expression tag |
Phe -2 | - | expression tag |
Gln -1 | - | expression tag |
Gly 0 | - | expression tag |
Pro 1 | - | expression tag |
Leu 360 | - | expression tag |
Glu 361 | - | expression tag |
Glu 362 | - | expression tag |
Asn 363 | - | expression tag |
Leu 364 | - | expression tag |
Tyr 365 | - | expression tag |
Phe 366 | - | expression tag |
Gln 367 | - | expression tag |
Gly 368 | - | expression tag |
His 369 | - | expression tag |
His 370 | - | expression tag |
His 371 | - | expression tag |
His 372 | - | expression tag |
His 373 | - | expression tag |
His 374 | - | expression tag |
His 375 | - | expression tag |
His 376 | - | expression tag |
His 377 | - | expression tag |
His 378 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 151228.2 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 543.9 | |
All* | Total formula weight | 151772.1 |