8YRQ

Cryo-EM Structure of Human Protease-Activated Receptor 4 in complex with Gq heterotrimers and ScFv16 bound to Tethered Ligand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Proteinase-activated receptor 4,Maltose/maltodextrin-binding periplasmic protein	polymer	714	77838.4	1	UniProt (Q96RI0) UniProt (P0AEX9)	Homo sapiens (human)	PAR-4,Coagulation factor II receptor-like 3,Thrombin receptor-like 3,MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
2	B (A)	Guanine nucleotide-binding protein mini G(o/q) subunit alpha	polymer	228	25634.3	1		Homo sapiens (human)
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	354	39086.6	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	81	9242.6	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
5	E (S)	ScFv16	polymer	303	32785.6	1		Homo sapiens (human)

Sequence modifications

R: 48 - 385 (UniProt: Q96RI0)

PDB	External Database	Details
Trp 165	Leu 165	conflict

R: 386 - 751 (UniProt: P0AEX9)

PDB	External Database	Details
Ala 557	Glu 198	conflict
Ala 558	Asn 199	conflict
Ala 624	Lys 265	conflict
His 752	-	expression tag
His 753	-	expression tag
His 754	-	expression tag
His 755	-	expression tag
His 756	-	expression tag
His 757	-	expression tag
His 758	-	expression tag
His 759	-	expression tag
His 760	-	expression tag
His 761	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

G: 2 - 71 (UniProt: P59768)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	184587.5
All*	Total formula weight	184587.5

*Water molecules are not included.