8YO4

structure of phage T4 topoisomerase II central domain bound with DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	DNA topoisomerase medium subunit	polymer	452	51952.0	2	UniProt (P07065) Pfam (PF00521)	Escherichia phage T4	DNA topoisomerase 51-kDa subunit,Protein Gp52
2	B, D (C, D)	phage T4 topoisomerase II gp39-gp60 subunit	polymer	682	77524.7	2	Pfam (PF02518) Pfam (PF00204) UniProt (by SIFTS) (P09176)	Escherichia phage T4
3	E (E)	DNA (5'-D(P*TP*GP*TP*GP*TP*GP*TP*AP*TP*AP*TP*AP*TP*AP*CP*AP*CP*AP*TP*AP*TP*AP*TP*A)-3')	polymer	52	16058.4	1		DNA molecule
4	F (F)	DNA (5'-D(P*AP*TP*AP*TP*AP*TP*GP*TP*GP*TP*AP*TP*AP*TP*AP*TP*AP*CP*AP*CP*AP*CP*AP*T)-3')	polymer	52	15965.4	1		DNA molecule
5	G, H, I, J (C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

A, B: 1 - 442 (UniProt: P07065)

PDB	External Database	Details
His 443	-	expression tag
His 444	-	expression tag
His 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag
His 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	290977.1
Non-Polymers*	Number of molecules	4
	Total formula weight	97.2
All*	Total formula weight	291074.3

*Water molecules are not included.