8YNT

Cryo-EM structure of SNAP-94847-bound MCHR1, S2 state

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (H)	Antibody 1B3 Fab heavy chain	polymer	238	25540.4	1		synthetic construct
2	B (L)	Antibody 1B3 Fab light chain	polymer	220	23796.4	1		synthetic construct
3	C (N)	Glue molecule 4-9	polymer	285	30972.2	1		synthetic construct
4	D (R)	Melanin-concentrating hormone receptor 1,Soluble cytochrome b562	polymer	585	64594.4	1	UniProt (Q5IFH6) UniProt (Q99705) UniProt (P0ABE7) UniProt (Q8CVG7) Pfam (PF00001) UniProt (by SIFTS) (A0A2R9AFP0)	Homo sapiens (human)	G-protein coupled receptor 24,MCH-1R,MCH receptor 1,MCH-R1,MCHR-1,G-protein coupled receptor 24,MCH-1R,MCH1R,MCHR,SLC-1,Somatostatin receptor-like protein,Cytochrome b-562
5	E (R)	~{N}-[3-[1-[[4-[3,4-bis(fluoranyl)phenoxy]phenyl]methyl]piperidin-4-yl]-4-methyl-phenyl]-2-methyl-propanamide	non-polymer		478.6	1	Chemie (A1D6T)

Sequence modifications

R: 1 - 69 (UniProt: Q5IFH6)

PDB	External Database	Details
Asp -45	-	expression tag
Tyr -44	-	expression tag
Lys -43	-	expression tag
Asp -42	-	expression tag
Asp -41	-	expression tag
Asp -40	-	expression tag
Asp -39	-	expression tag
Met -38	-	expression tag
Gly -37	-	expression tag
Gln -36	-	expression tag
Pro -35	-	expression tag
Gly -34	-	expression tag
Asn -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ala -30	-	expression tag
Phe -29	-	expression tag
Leu -28	-	expression tag
Leu -27	-	expression tag
Ala -26	-	expression tag
Pro -25	-	expression tag
Asn -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
His -21	-	expression tag
Ala -20	-	expression tag
Pro -19	-	expression tag
Asp -18	-	expression tag
His -17	-	expression tag
Asp -16	-	expression tag
Val -15	-	expression tag
Thr -14	-	expression tag
Gln -13	-	expression tag
Gln -12	-	expression tag
Arg -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Val -1	-	expression tag
Asp 0	-	expression tag
Asn 32	Asp 32	conflict

R: 70 - 304 (UniProt: Q99705)
R: 309 - 351 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 315	Met 29	conflict
Ser 352	-	linker
Gly 353	-	linker
Ser 354	-	linker
Gly 355	-	linker

R: 356 - 402 (UniProt: Q8CVG7)

PDB	External Database	Details
Ile 403	-	linker
Gln 404	-	linker
Lys 405	-	linker
Tyr 406	-	linker
Leu 407	-	linker
Leu 408	-	linker

R: 409 - 489 (UniProt: Q99705)

PDB	External Database	Details
Lys 305	-	linker
Arg 306	-	linker
Gln 307	-	linker
Leu 308	-	linker
Ile 490	-	expression tag
Ala 491	-	expression tag
Ala 492	-	expression tag
Leu 493	-	expression tag
Lys 494	-	expression tag
Glu 495	-	expression tag
Lys 496	-	expression tag
Ile 497	-	expression tag
Ala 498	-	expression tag
Ala 499	-	expression tag
Leu 500	-	expression tag
Lys 501	-	expression tag
Glu 502	-	expression tag
Lys 503	-	expression tag
Ile 504	-	expression tag
Ala 505	-	expression tag
Ala 506	-	expression tag
Leu 507	-	expression tag
Lys 508	-	expression tag
Glu 509	-	expression tag
Ala 510	-	expression tag
Glu 511	-	expression tag
Glu 512	-	expression tag
Lys 513	-	expression tag
Arg 514	-	expression tag
Ala 515	-	expression tag
Ser 516	-	expression tag
Arg 517	-	expression tag
Leu 518	-	expression tag
Glu 519	-	expression tag
Glu 520	-	expression tag
Glu 521	-	expression tag
Leu 522	-	expression tag
Arg 523	-	expression tag
Arg 524	-	expression tag
Arg 525	-	expression tag
Leu 526	-	expression tag
Thr 527	-	expression tag
Glu 528	-	expression tag
Ala 529	-	expression tag
Ala 530	-	expression tag
Ala 531	-	expression tag
His 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	144903.4
Non-Polymers*	Number of molecules	1
	Total formula weight	478.6
All*	Total formula weight	145382.0

*Water molecules are not included.