8Y7Y
Local structure of HCoV-HKU1A spike in complex with TMPRSS2 and glycan
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Spike glycoprotein | polymer | 1268 | 141235.3 | 2 | UniProt (Q5MQD0) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB | Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) | S glycoprotein,E2,Peplomer protein |
| 2 | T | Transmembrane protease serine 2 | polymer | 383 | 42283.6 | 1 | UniProt (O15393) Pfam (PF15494) Pfam (PF00089) In PDB | Homo sapiens (human) | Serine protease 10 |
| 3 | C, D | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 2 | In PDB GlyTouCan (G81315DD) | |||
| 4 | E, I | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 5 | F, G, H, J | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 6 | A, B | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) | |||
| 7 | A | methyl 9-O-acetyl-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosidonic acid | non-polymer | 365.3 | 1 | Chemie (MJJ) |
Sequence modifications
A, B: 14 - 1281 (UniProt: Q5MQD0)
T: 110 - 492 (UniProt: O15393)
| PDB | External Database | Details |
|---|---|---|
| Gly 756 | Arg 756 | engineered mutation |
| Gly 757 | Arg 757 | engineered mutation |
| Ser 758 | Lys 758 | engineered mutation |
| Gly 759 | Arg 759 | engineered mutation |
| Ser 760 | Arg 760 | engineered mutation |
| Pro 1071 | Ala 1071 | engineered mutation |
| Pro 1072 | Leu 1072 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asp 251 | Ser 250 | engineered mutation |
| Asp 252 | Ser 251 | engineered mutation |
| - | Arg 252 | deletion |
| Asp 253 | Gln 253 | engineered mutation |
| Asp 254 | Ser 254 | engineered mutation |
| Lys 255 | Arg 255 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 324754.3 | |
| Branched | Number of molecules | 8 |
| Total formula weight | 4368.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1250.2 | |
| All* | Total formula weight | 330372.5 |
