8Y7X

Structure of HCoV-HKU1A spike in the functionally anchored-3up conformation with 3TMPRSS2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (B, A, C)	Spike glycoprotein	polymer	1268	141235.3	3	UniProt (Q5MQD0)	Human coronavirus HKU1 (isolate N1) (HCoV-HKU1)	S glycoprotein,E2,Peplomer protein
2	D, E, F (x, y, z)	Transmembrane protease serine 2	polymer	383	42283.6	3	UniProt (O15393) Pfam (PF15494) Pfam (PF00089)	Homo sapiens (human)	Serine protease 10
3	G, H, I, J, K... (D, E, F, G, H...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	10	In PDB GlyTouCan (G42666HT)
4	AA, BA, CA, DA, EA... (A, C, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	23	Chemie (NAG)

Sequence modifications

B, A, C: 14 - 1281 (UniProt: Q5MQD0)

PDB	External Database	Details
Gly 756	Arg 756	engineered mutation
Gly 757	Arg 757	engineered mutation
Ser 758	Lys 758	engineered mutation
Gly 759	Arg 759	engineered mutation
Ser 760	Arg 760	engineered mutation
Pro 1071	Ala 1071	engineered mutation
Pro 1072	Leu 1072	engineered mutation

x, y, z: 110 - 492 (UniProt: O15393)

PDB	External Database	Details
Asp 251	Ser 250	engineered mutation
Asp 252	Ser 251	engineered mutation
-	Arg 252	deletion
Asp 253	Gln 253	engineered mutation
Asp 254	Ser 254	engineered mutation
Lys 255	Arg 255	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	550556.9
Branched	Number of molecules	10
	Total formula weight	4244.0
Non-Polymers*	Number of molecules	23
	Total formula weight	5087.8
All*	Total formula weight	559888.7

*Water molecules are not included.