8Y5H
Cryo-EM structure of E.coli spermidine transporter PotD-PotABC in pre-translocation state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Spermidine/putrescine import ATP-binding protein PotA | polymer | 378 | 43079.1 | 2 | UniProt (A0A1Q6AZ03) Pfam (PF00005) Pfam (PF08402) UniProt (by SIFTS) (P69874) | Escherichia coli | |
| 2 | B (B) | Spermidine/putrescine ABC transporter membrane protein | polymer | 285 | 32234.2 | 1 | UniProt (A0A037Y861) Pfam (PF00528) UniProt (by SIFTS) (P0AFK4) | Escherichia coli | |
| 3 | C (C) | Spermidine/putrescine transport system permease protein PotC | polymer | 264 | 29132.8 | 1 | UniProt (C3TDI7) Pfam (PF00528) UniProt (by SIFTS) (P0AFK6) | Escherichia coli | |
| 4 | E (E) | Spermidine/putrescine-binding periplasmic protein | polymer | 348 | 38906.9 | 1 | UniProt (P0AFK9) Pfam (PF13416) | Escherichia coli | SPBP |
| 5 | F (E) | SPERMIDINE | non-polymer | 145.2 | 1 | Chemie (SPD) | |||
| 6 | G (C) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A, D: 1 - 378 (UniProt: A0A1Q6AZ03)
| PDB | External Database | Details |
|---|---|---|
| Gln 173 | Glu 173 | engineered mutation |
| Asp 223 | Glu 223 | conflict |
| His 377 | Leu 377 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 186432.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 145.2 | |
| All* | Total formula weight | 186577.5 |
