8Y5H

Cryo-EM structure of E.coli spermidine transporter PotD-PotABC in pre-translocation state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0005524	molecular_function	ATP binding
A	0005886	cellular_component	plasma membrane
A	0015417	molecular_function	ABC-type polyamine transporter activity
A	0015594	molecular_function	ABC-type putrescine transporter activity
A	0015846	biological_process	polyamine transport
A	0015847	biological_process	putrescine transport
A	0016020	cellular_component	membrane
A	0016887	molecular_function	ATP hydrolysis activity
A	0022857	molecular_function	transmembrane transporter activity
A	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
A	0055085	biological_process	transmembrane transport
A	1903711	biological_process	spermidine transmembrane transport
B	0005886	cellular_component	plasma membrane
B	0015417	molecular_function	ABC-type polyamine transporter activity
B	0015847	biological_process	putrescine transport
B	0016020	cellular_component	membrane
B	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
B	0055085	biological_process	transmembrane transport
B	1903711	biological_process	spermidine transmembrane transport
C	0005886	cellular_component	plasma membrane
C	0015417	molecular_function	ABC-type polyamine transporter activity
C	0015847	biological_process	putrescine transport
C	0016020	cellular_component	membrane
C	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
C	0055085	biological_process	transmembrane transport
C	1903711	biological_process	spermidine transmembrane transport
D	0000166	molecular_function	nucleotide binding
D	0005524	molecular_function	ATP binding
D	0005886	cellular_component	plasma membrane
D	0015417	molecular_function	ABC-type polyamine transporter activity
D	0015594	molecular_function	ABC-type putrescine transporter activity
D	0015846	biological_process	polyamine transport
D	0015847	biological_process	putrescine transport
D	0016020	cellular_component	membrane
D	0016887	molecular_function	ATP hydrolysis activity
D	0022857	molecular_function	transmembrane transporter activity
D	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
D	0055085	biological_process	transmembrane transport
D	1903711	biological_process	spermidine transmembrane transport
E	0015846	biological_process	polyamine transport
E	0019808	molecular_function	polyamine binding
E	0042597	cellular_component	periplasmic space

Functional Information from PROSITE/UniProt

site_id	PS00211
Number of Residues	15
Details	ABC_TRANSPORTER_1 ABC transporters family signature. LSGGQQQRVAIARAV

Chain	Residue	Details
A	LEU148-VAL162

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	59
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
C	MET1-ARG7
C	TYR86-LEU100
C	SER149-ILE176
C	ALA252-LYS264
B	ILE198-ALA218
B	PHE252-TRP272

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site_id	SWS_FT_FI2
Number of Residues	114
Details	TRANSMEM: Helical => ECO:0000255|PROSITE-ProRule:PRU00441

Chain	Residue	Details
C	GLY8-VAL27
C	MET66-LEU85
C	PHE101-MET120
C	TRP129-TYR148
C	ILE177-SER196
C	ALA232-ILE251

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site_id	SWS_FT_FI3
Number of Residues	78
Details	TOPO_DOM: Periplasmic => ECO:0000255

Chain	Residue	Details
C	ASN28-THR65
C	LEU121-PHE128
C	MET197-ASN231

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