8Y2O

The Cryo-EM structure of human tRNA methyltransferase FTSJ1-THADA with substrate tRNA and S-adenosyl homocysteine (SAH)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	tRNA (32-2'-O)-methyltransferase regulator THADA	polymer	1981	223138.8	1	UniProt (Q6YHU6) Pfam (PF10350)	Homo sapiens (human)	Gene inducing thyroid adenomas protein,Thyroid adenoma-associated protein
2	B (B)	tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase	polymer	347	38224.2	1	UniProt (Q9UET6) Pfam (PF01728)	Homo sapiens (human)	2'-O-ribose RNA methyltransferase TRM7 homolog,Protein ftsJ homolog 1
3	C (C)	human cytoplasmic tRNA(Phe)	polymer	76	24518.6	1	GenBank (29540470)	Homo sapiens
4	D (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	E (B)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
6	F, G, H, I, J... (C)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)

Sequence modifications

A: 1 - 1953 (UniProt: Q6YHU6)

PDB	External Database	Details
Met -27	-	initiating methionine
Asp -26	-	expression tag
Tyr -25	-	expression tag
Lys -24	-	expression tag
Asp -23	-	expression tag
Asp -22	-	expression tag
Asp -21	-	expression tag
Asp -20	-	expression tag
Lys -19	-	expression tag
Ser -18	-	expression tag
Ala -17	-	expression tag
Trp -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Pro -13	-	expression tag
Gln -12	-	expression tag
Phe -11	-	expression tag
Glu -10	-	expression tag
Lys -9	-	expression tag
Gly -8	-	expression tag
Ala -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

B: 1 - 329 (UniProt: Q9UET6)

PDB	External Database	Details
Met -17	-	initiating methionine
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Gly -8	-	expression tag
Ala -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

C: 1 - 73 (GenBank: 29540470)

PDB	External Database	Details
C 74	-	insertion
C 75	-	insertion
A 76	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	285881.6
Non-Polymers*	Number of molecules	9
	Total formula weight	620.0
All*	Total formula weight	286501.6

*Water molecules are not included.