8XWC
Crystal structure of human 8-oxoguanine glycosylase K249H mutant bound to the substrate 8-oxoguanine DNA at pH 8.0 under 277 K
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | DNA (5'-D(*AP*GP*CP*GP*TP*CP*CP*AP*(8OG)P*GP*TP*CP*TP*AP*CP*C)-3') | polymer | 16 | 4875.2 | 1 | Homo sapiens | ||
| 2 | B (D) | DNA (5'-D(P*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3') | polymer | 15 | 4635.0 | 1 | Homo sapiens | ||
| 3 | C (A) | N-glycosylase/DNA lyase | polymer | 336 | 37700.6 | 1 | UniProt (O15527) | Homo sapiens (human) | |
| 4 | D (D) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 5 | E, F, G (D, A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 6 | H (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | I (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 8 | J, K, L (C, D, A) | water | water | 18.0 | 433 | Chemie (HOH) |
Sequence modifications
A: 12 - 345 (UniProt: O15527)
| PDB | External Database | Details |
|---|---|---|
| Gly 10 | - | expression tag |
| Pro 11 | - | expression tag |
| His 249 | Lys 249 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 47210.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 429.7 | |
| All* | Total formula weight | 47640.5 |
