8XVH

Cryo-EM structure of ETBR bound with Endothelin1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	261	30464.3	1		Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38045.6	1	UniProt (P62873) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) In PDB	Homo sapiens (human)	G gamma-I
4	N	Nanobody 35	polymer	157	17057.3	1		Lama glama
5	T	Endothelin-1	polymer	21	2498.0	1	UniProt (P05305) In PDB	Homo sapiens (human)	ET-1
6	R	Exo-alpha-sialidase,Endothelin receptor type B	polymer	837	94123.8	1	UniProt (Q59310) UniProt (P24530) In PDB	Clostridium perfringens	ET-B,ET-BR,Endothelin receptor non-selective type

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Ile -5	-	expression tag
Gly -4	-	expression tag
Arg -3	-	expression tag
Ala -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Phe 1	-	expression tag

R: -385 - 66 (UniProt: Q59310)

PDB	External Database	Details
Met -412	-	initiating methionine
Lys -411	-	expression tag
Thr -410	-	expression tag
Ile -409	-	expression tag
Ile -408	-	expression tag
Ala -407	-	expression tag
Leu -406	-	expression tag
Ser -405	-	expression tag
Tyr -404	-	expression tag
Ile -403	-	expression tag
Phe -402	-	expression tag
Cys -401	-	expression tag
Leu -400	-	expression tag
Val -399	-	expression tag
Phe -398	-	expression tag
Ala -397	-	expression tag
Asp -396	-	expression tag
Tyr -395	-	expression tag
Lys -394	-	expression tag
Asp -393	-	expression tag
Asp -392	-	expression tag
Asp -391	-	expression tag
Asp -390	-	expression tag
Ala -389	-	expression tag
Gly -388	-	expression tag
Arg -387	-	expression tag
Ala -386	-	expression tag
Ser -235	Gly 393	conflict

R: 67 - 406 (UniProt: P24530)

PDB	External Database	Details
Leu 407	-	expression tag
Glu 408	-	expression tag
Val 409	-	expression tag
Leu 410	-	expression tag
Phe 411	-	expression tag
Gln 412	-	expression tag
Gly 413	-	expression tag
Pro 414	-	expression tag
His 415	-	expression tag
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	190050.1
All*	Total formula weight	190050.1

*Water molecules are not included.