8XUD

Crystal structure of adaptor NlpI in complex with endopeptidase MepS and PDZ-protease Prc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Lipoprotein NlpI	polymer	297	34082.7	2	UniProt (P0AFB1)	Escherichia coli K-12
2	C, D (C, D)	Tail-specific protease	polymer	688	77520.8	2	UniProt (P23865) Pfam (PF17804) Pfam (PF00595) Pfam (PF03572) Pfam (PF11818)	Escherichia coli K-12	C-terminal-processing peptidase,PRC protein,Protease Re
3	E, F, G, H (I, J, K, L)	Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase	polymer	168	19148.4	4	UniProt (P0AFV4) Pfam (PF00877)	Escherichia coli K-12	Lipoprotein Spr,Murein hydrolase MepS
4	I (M)	Substrate peptide	polymer	8	698.9	1		Escherichia coli K-12
5	J (N)	Substrate peptide	polymer	9	784.0	1		Escherichia coli K-12
6	K, L, M, N, O... (I, J, K, L)	PHOSPHATE ION	non-polymer		95.0	14	Chemie (PO4)

Sequence modifications

A, B: 20 - 294 (UniProt: P0AFB1)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
Gly 10	-	expression tag
Glu 11	-	expression tag
Asn 12	-	expression tag
Leu 13	-	expression tag
Tyr 14	-	expression tag
Phe 15	-	expression tag
Gln 16	-	expression tag
Gly 17	-	expression tag
His 18	-	expression tag
Met 19	-	expression tag

C, D: 1 - 682 (UniProt: P23865)

PDB	External Database	Details
Ala 452	Ser 452	engineered mutation
Ala 477	Lys 477	engineered mutation
His 683	-	expression tag
His 684	-	expression tag
His 685	-	expression tag
His 686	-	expression tag
His 687	-	expression tag
His 688	-	expression tag

I, J, K, L: 2 - 162 (UniProt: P0AFV4)

PDB	External Database	Details
Met 1	-	initiating methionine
His 163	-	expression tag
His 164	-	expression tag
His 165	-	expression tag
His 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	301283.2
Non-Polymers*	Number of molecules	14
	Total formula weight	1329.6
All*	Total formula weight	302612.8

*Water molecules are not included.