8XRP

The Cryo-EM structure of IL-12, receptor subunit beta-1 and receptor subunit beta-2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (A, E, I, M)	Interleukin-12 subunit alpha	polymer	207	23781.5	4	UniProt (P29459) Pfam (PF03039)	Homo sapiens (human)	IL-12A,Cytotoxic lymphocyte maturation factor 35 kDa subunit,CLMF p35,IL-12 subunit p35,NK cell stimulatory factor chain 1,NKSF1
2	B, F, J, N (B, F, J, N)	Interleukin-12 subunit beta	polymer	307	34811.0	4	UniProt (P29460) Pfam (PF10420)	Homo sapiens (human)	IL-12B,Cytotoxic lymphocyte maturation factor 40 kDa subunit,CLMF p40,IL-12 subunit p40,NK cell stimulatory factor chain 2,NKSF2
3	C, G, K, O (C, G, K, O)	Interleukin-12 receptor subunit beta-2	polymer	302	34627.5	4	UniProt (Q99665) Pfam (PF06328)	Homo sapiens (human)	IL-12 receptor subunit beta-2,IL-12R subunit beta-2,IL-12R-beta-2,IL-12RB2
4	D, H, L, P (D, H, L, P)	Interleukin-12 receptor subunit beta-1	polymer	219	24736.4	4	UniProt (P42701)	Homo sapiens (human)	IL-12 receptor subunit beta-1,IL-12R subunit beta-1,IL-12R-beta-1,IL-12RB1,IL-12 receptor beta component
5	Q, R, S, T (Q, R, S, T)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G15407YE)
6	AA, BA, CA, DA, EA... (K, O, C, G)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	12	Chemie (NAG)

Sequence modifications

A, E, I, M: 19 - 219 (UniProt: P29459)

PDB	External Database	Details
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag

C, G, K, O: 24 - 319 (UniProt: Q99665)

PDB	External Database	Details
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag

D, H, L, P: 24 - 236 (UniProt: P42701)

PDB	External Database	Details
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	471825.8
Branched	Number of molecules	4
	Total formula weight	2346.2
Non-Polymers*	Number of molecules	12
	Total formula weight	2654.5
All*	Total formula weight	476826.5

*Water molecules are not included.