8XKF

Crystal structure of Helicobacter pylori IspDF with substrate CTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Bifunctional enzyme IspD/IspF	polymer	381	42890.3	6	UniProt (O25664) Pfam (PF01128) Pfam (PF02542) In PDB	Helicobacter pylori 26695
2	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3	E, A, B, D	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
4	E, F, A, B, C...	CYTIDINE-5'-TRIPHOSPHATE	non-polymer		483.2	6	Chemie (CTP)
5	F, A, C, D	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
6	A, D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	F, B, D	CYTIDINE-5'-MONOPHOSPHATE	non-polymer		323.2	3	Chemie (C5P)
8	E, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
9	E, C	CYTIDINE-5'-DIPHOSPHATE	non-polymer		403.2	2	Chemie (CDP)
10		water	water		18.0	673	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 33 - 406 (UniProt: O25664)

PDB	External Database	Details
Met 26	-	initiating methionine
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	257341.7
Non-Polymers*	Number of molecules	27
	Total formula weight	5866.2
All*	Total formula weight	263207.9

*Water molecules are not included.