8XI2

Cryo-EM structure of the Chlamydomonas C* complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	MPN domain-containing protein	polymer	2398	278543.4	1	UniProt (A0A2K3E5Q8) In PDB	Chlamydomonas reinhardtii
2	B	U5 snRNA	polymer	111	35370.8	1		Chlamydomonas reinhardtii
3	C	Elongation factor Tu, chloroplastic	polymer	989	109366.8	1	UniProt (A8JCA8) In PDB	Chlamydomonas reinhardtii	Snu114
4	E	U5-40K	polymer	362	39437.4	1	UniProt (A0A2K3DMA3) In PDB	Chlamydomonas reinhardtii
5	F	U6 snRNA	polymer	101	32760.4	1		Chlamydomonas reinhardtii
6	q, r, s, t	Pre-mRNA-processing factor 19	polymer	503	54015.8	4	UniProt (A8I9S6) In PDB	Chlamydomonas reinhardtii
7	a	Small nuclear ribonucleoprotein Sm D3	polymer	127	13789.2	1	UniProt (A8I0E2) In PDB	Chlamydomonas reinhardtii	Sm-D3,snRNP core protein D3
8	g	Small nuclear ribonucleoprotein G	polymer	77	8533.0	1	UniProt (A8HRS8) In PDB	Chlamydomonas reinhardtii	snRNP-G
9	e	Small nuclear ribonucleoprotein E	polymer	87	10216.1	1	UniProt (A8IYZ2) In PDB	Chlamydomonas reinhardtii	snRNP-E,Sm protein E
10	f	Sm protein F	polymer	85	9644.1	1	UniProt (A8J834) In PDB	Chlamydomonas reinhardtii
11	d	Small nuclear ribonucleoprotein Sm D2	polymer	110	12630.7	1	UniProt (A8I074) In PDB	Chlamydomonas reinhardtii	Sm-D2,snRNP core protein D2
12	c	Small nuclear ribonucleoprotein Sm D1	polymer	114	12668.9	1	UniProt (A0A2K3CTT9) In PDB	Chlamydomonas reinhardtii	snRNP core protein D1
13	b	Sm domain-containing protein	polymer	265	27745.2	1	UniProt (A0A2K3CX88) In PDB	Chlamydomonas reinhardtii
14	I	Syf1	polymer	925	104377.6	1	UniProt (A0A2K3D3K4) In PDB	Chlamydomonas reinhardtii
15	J	Crooked neck protein	polymer	835	96708.7	1	UniProt (A0A2K3CXZ4) In PDB	Chlamydomonas reinhardtii
16	P	Cwf15/Cwc15 cell cycle control protein	polymer	235	26422.3	1	UniProt (A0A2K3CN43) In PDB	Chlamydomonas reinhardtii
17	M	PPIase cyclophilin-type domain-containing protein	polymer	563	61094.6	1	UniProt (A0A2K3E769) In PDB	Chlamydomonas reinhardtii
18	T	PLRG1	polymer	518	56100.5	1	UniProt (A0A2K3DAW8) In PDB	Chlamydomonas reinhardtii
19	O	Rbm22	polymer	417	44413.2	1	UniProt (A0A2K3E050) In PDB	Chlamydomonas reinhardtii
20	N	G10 protein	polymer	233	26355.9	1	UniProt (A0A2K3DAL7) In PDB	Chlamydomonas reinhardtii	Bud31
21	R	SKI-interacting protein SKIP SNW domain-containing protein	polymer	684	73924.8	1	UniProt (A0A2K3E831) In PDB	Chlamydomonas reinhardtii
22	H	U2 snRNA	polymer	192	61386.1	1	GenBank (2826764)	Chlamydomonas reinhardtii
23	S	Peptidyl-prolyl cis-trans isomerase	polymer	157	17309.7	1	UniProt (A8JGI0) In PDB	Chlamydomonas reinhardtii	PPIase
24	W	Prp17	polymer	576	63995.9	1	UniProt (A0A2K3DUN6) In PDB	Chlamydomonas reinhardtii
25	L	Cdc5L	polymer	833	91838.6	1	UniProt (A0A2K3DYR4) In PDB	Chlamydomonas reinhardtii
26	K	Pre-mRNA-splicing factor SPF27	polymer	303	32563.0	1	UniProt (A0A2K3CNP4) In PDB	Chlamydomonas reinhardtii
27	U	CWF21 domain-containing protein	polymer	721	80630.3	1	UniProt (A0A2K3DFH0) In PDB	Chlamydomonas reinhardtii
28	V	MI domain-containing protein	polymer	928	102389.4	1	UniProt (A0A2K3DML4) In PDB	Chlamydomonas reinhardtii	Cwc22
29	Q	Intron-binding protein aquarius	polymer	1844	201451.0	1	UniProt (A0A2K3DR41) In PDB	Chlamydomonas reinhardtii
30	5	RNA (5'-R(P*CP*CP*GP*AP*AP*CP*G)-3')	polymer	7	2219.4	1		Chlamydomonas reinhardtii
31	3	RNA (173-mer)	polymer	173	55292.4	1		Chlamydomonas reinhardtii
32	C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
33	C, F	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
34	O, N	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	34
	Total formula weight	2005242.5
Non-Polymers*	Number of molecules	11
	Total formula weight	889.5
All*	Total formula weight	2006132.1

*Water molecules are not included.