8X83

The cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster in complex with fructose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Gustatory receptor for sugar taste 43a	polymer	443	49267.3	4	UniProt (Q9V4K2) Pfam (PF08395)	Drosophila melanogaster (fruit fly)
2	E, F (A)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
3	G, H, I, J (A, B, C, D)	beta-D-fructofuranose	non-polymer		180.2	4	Chemie (FRU)
4	K, L, M, N (A, B, C, D)	water	water		18.0	4	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 427 (UniProt: Q9V4K2)

PDB	External Database	Details
Ala 418	Ile 418	engineered mutation
Leu 428	-	expression tag
Glu 429	-	expression tag
Gly 430	-	expression tag
Gly 431	-	expression tag
Ser 432	-	expression tag
Ser 433	-	expression tag
Gly 434	-	expression tag
Gly 435	-	expression tag
Trp 436	-	expression tag
Ser 437	-	expression tag
His 438	-	expression tag
Pro 439	-	expression tag
Gln 440	-	expression tag
Phe 441	-	expression tag
Glu 442	-	expression tag
Lys 443	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	197069.2
Non-Polymers*	Number of molecules	6
	Total formula weight	766.6
All*	Total formula weight	197835.9

*Water molecules are not included.