8X52

Cryo-EM structure of human gamma-secretase in complex with Abeta49

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E	Amyloid-beta precursor protein	polymer	120	13800.6	1	UniProt (P05067) Pfam (PF03494) Pfam (PF10515) In PDB	Homo sapiens (human)	APP,ABPP,APPI,Alzheimer disease amyloid A4 protein homolog,Alzheimer disease amyloid protein,Amyloid precursor protein,Amyloid-beta (A4) precursor protein,Amyloid-beta A4 protein,Cerebral vascular amyloid peptide,CVAP,PreA4,Protease nexin-II,PN-II
2	A	Nicastrin	polymer	709	78473.6	1	UniProt (Q92542) Pfam (PF18266) Pfam (PF05450) In PDB	Homo sapiens (human)
3	B	Presenilin-1	polymer	467	52712.6	1	UniProt (P49768) Pfam (PF01080) In PDB	Homo sapiens (human)	PS-1,Protein S182
4	C	Gamma-secretase subunit APH-1A	polymer	265	29017.9	1	UniProt (Q96BI3) Pfam (PF06105) In PDB	Homo sapiens (human)	APH-1a,Aph-1alpha,Presenilin-stabilization factor
5	D	Gamma-secretase subunit PEN-2	polymer	101	12038.0	1	UniProt (Q9NZ42) Pfam (PF10251) In PDB	Homo sapiens (human)	Presenilin enhancer protein 2
6	F, H, I, J, K	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
7	G	beta-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G89225LT)
8	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
9	B, C	1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		790.1	2	Chemie (PC1)
10	C	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)

Sequence modifications

E: 0 - 99 (UniProt: P05067)

PDB	External Database	Details
Cys 28	Lys 568	conflict
Glu 100	-	expression tag
Gln 101	-	expression tag
Lys 102	-	expression tag
Leu 103	-	expression tag
Ile 104	-	expression tag
Ser 105	-	expression tag
Glu 106	-	expression tag
Glu 107	-	expression tag
Asp 108	-	expression tag
Leu 109	-	expression tag
Leu 110	-	expression tag
Glu 111	-	expression tag
His 112	-	expression tag
His 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag

A: 1 - 709 (UniProt: Q92542)

PDB	External Database	Details
Cys 242	Ile 242	conflict

B: 1 - 467 (UniProt: P49768)

PDB	External Database	Details
Asn 385	Asp 385	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	186042.7
Branched	Number of molecules	6
	Total formula weight	3032.8
Non-Polymers*	Number of molecules	10
	Total formula weight	3680.8
All*	Total formula weight	192756.4

*Water molecules are not included.