8X4L

Crystal structure of the N132A mutant of DIMT1 from Pyrococcus horikoshii

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Probable ribosomal RNA small subunit methyltransferase A	polymer	290	33322.1	2	UniProt (O59487) Pfam (PF00398)	Pyrococcus horikoshii OT3
2	C, D, E, F, P... (A, B)	ZINC ION	non-polymer		65.4	7	Chemie (ZN)
3	G, H, I, J, K... (A, B)	SULFITE ION	non-polymer		80.1	6	Chemie (SO3)
4	L, M, N, V, W (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
5	O, X (A, B)	ARGININE	non-polymer		175.2	2	Chemie (ARG)
6	T (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7	U (B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
8	Y, Z (A, B)	water	water		18.0	115	Chemie (HOH)

Sequence modifications

A, B: 23 - 290 (UniProt: O59487)

PDB	External Database	Details
Met 1	-	initiating methionine
Ser 2	-	expression tag
Ser 3	-	expression tag
Arg 4	-	expression tag
Ile 5	-	expression tag
Arg 6	-	expression tag
Ile 7	-	expression tag
Asn 8	-	expression tag
Thr 9	-	expression tag
Lys 10	-	expression tag
Ser 11	-	expression tag
Leu 12	-	expression tag
Leu 13	-	expression tag
Val 14	-	expression tag
Gln 15	-	expression tag
Pro 16	-	expression tag
Gly 17	-	expression tag
Tyr 18	-	expression tag
Ser 19	-	expression tag
Gly 20	-	expression tag
Ser 21	-	expression tag
Lys 22	-	expression tag
Ala 132	Asn 110	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66644.2
Non-Polymers*	Number of molecules	22
	Total formula weight	1797.2
All*	Total formula weight	68441.4

*Water molecules are not included.