8X2K

OEA bound GPR3-Gs complex structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Soluble cytochrome b562,G-protein coupled receptor 3	polymer	466	50446.4	1	UniProt (P0ABE7) UniProt (P46089) Pfam (PF07361) Pfam (PF00001)	Homo sapiens (human)	Cytochrome b-562
2	B (B)	Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	373	43385.2	1	UniProt (P63092-2) Pfam (PF00503) UniProt (by SIFTS) (P63092)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	357	39286.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
4	D (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (E)	scfv16	polymer	261	27691.9	1		Homo sapiens
6	F (A)	(Z)-N-(2-hydroxyethyl)octadec-9-enamide	non-polymer		325.5	1	Chemie (5YM)
7	G (A)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A: -105 - -1 (UniProt: P0ABE7)

PDB	External Database	Details
Asp -123	-	expression tag
Tyr -122	-	expression tag
Lys -121	-	expression tag
Asp -120	-	expression tag
Asp -119	-	expression tag
Asp -118	-	expression tag
Asp -117	-	expression tag
Lys -116	-	expression tag
Leu -115	-	expression tag
Glu -114	-	expression tag
Val -113	-	expression tag
Leu -112	-	expression tag
Phe -111	-	expression tag
Gln -110	-	expression tag
Gly -109	-	expression tag
Pro -108	-	expression tag
Gly -107	-	expression tag
Ser -106	-	expression tag
Trp -99	Met 29	conflict
Ile -4	His 124	conflict
Leu 0	-	linker

A: 1 - 330 (UniProt: P46089)

PDB	External Database	Details
Leu 331	-	expression tag
Glu 332	-	expression tag
His 333	-	expression tag
His 334	-	expression tag
His 335	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag

B: 26 - 394 (UniProt: P63092-2)

PDB	External Database	Details
Met 8	-	initiating methionine
Gly 9	-	expression tag
Cys 10	-	expression tag
Thr 11	-	expression tag
Leu 12	-	expression tag
Ser 13	-	expression tag
Ala 14	-	expression tag
Glu 15	-	expression tag
Asp 16	-	expression tag
Lys 17	-	expression tag
Ala 18	-	expression tag
Ala 19	-	expression tag
Val 20	-	expression tag
Glu 21	-	expression tag
Arg 22	-	expression tag
Ser 23	-	expression tag
Lys 24	-	expression tag
Met 25	-	expression tag

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	168671.6
Non-Polymers*	Number of molecules	1
	Total formula weight	325.5
All*	Total formula weight	168997.1

*Water molecules are not included.