8X20

HIV-1 reverse transcriptase mutant Q151M/Y115F/F116Y/L74V:DNA:E-CFCP-TP ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Pol protein (Fragment)	polymer	557	64019.4	2	UniProt (D3XFN5) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) UniProt (by SIFTS) (P12497) In PDB	Human immunodeficiency virus 1
2	B, D	HIV-1 RT p51 subunit	polymer	444	51861.5	2	UniProt (P12497) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB	Human immunodeficiency virus 1	HIV-1 reverse transcriptase p51 subunit, p51 RT
3	E, F	DNA/RNA (38-MER)	polymer	38	11724.5	2		synthetic construct
4	A, C	E-CFCP-triphosphate	non-polymer		560.2	2	Chemie (XTE)
5	B, E, C, D	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
6	C	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7		water	water		18.0	248	Chemie (HOH)

Sequence modifications

A, C: 1 - 555 (UniProt: D3XFN5)

PDB	External Database	Details
Met -1	-	initiating methionine
Val 0	-	expression tag
Val 74	Leu 173	engineered mutation
Phe 115	Tyr 214	engineered mutation
Tyr 116	Phe 215	engineered mutation
Met 151	Gln 250	engineered mutation
Ser 162	Cys 261	engineered mutation
Ser 280	Cys 379	engineered mutation

B, D: 1 - 428 (UniProt: P12497)

PDB	External Database	Details
Met -15	-	expression tag
Ala -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ala -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ser 162	Cys 749	engineered mutation
Ser 280	Cys 867	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	255210.8
Non-Polymers*	Number of molecules	8
	Total formula weight	1605.2
All*	Total formula weight	256816.0

*Water molecules are not included.