8X20
HIV-1 reverse transcriptase mutant Q151M/Y115F/F116Y/L74V:DNA:E-CFCP-TP ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, C) | Pol protein (Fragment) | polymer | 557 | 64019.4 | 2 | UniProt (D3XFN5) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) UniProt (by SIFTS) (P12497) | Human immunodeficiency virus 1 | |
| 2 | B, E (B, D) | HIV-1 RT p51 subunit | polymer | 444 | 51861.5 | 2 | UniProt (P12497) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) | Human immunodeficiency virus 1 | HIV-1 reverse transcriptase p51 subunit, p51 RT |
| 3 | C, F (E, F) | DNA/RNA (38-MER) | polymer | 38 | 11724.5 | 2 | synthetic construct | ||
| 4 | G, J (A, C) | E-CFCP-triphosphate | non-polymer | 560.2 | 2 | Chemie (XTE) | |||
| 5 | H, I, L, M, N (B, E, C, D) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 6 | K (C) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | O, P, Q, R, S... (A, B, E, C, D...) | water | water | 18.0 | 248 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 555 (UniProt: D3XFN5)
B, D: 1 - 428 (UniProt: P12497)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Val 0 | - | expression tag |
| Val 74 | Leu 173 | engineered mutation |
| Phe 115 | Tyr 214 | engineered mutation |
| Tyr 116 | Phe 215 | engineered mutation |
| Met 151 | Gln 250 | engineered mutation |
| Ser 162 | Cys 261 | engineered mutation |
| Ser 280 | Cys 379 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | expression tag |
| Ala -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Val -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ser 162 | Cys 749 | engineered mutation |
| Ser 280 | Cys 867 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 255210.8 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1605.2 | |
| All* | Total formula weight | 256816.0 |
