8WST

Cryo-EM structure of Melanin-Concentrating Hormone Receptor 2 with MCH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(q) subunit alpha-1	polymer	246	28084.8	1		Homo sapiens
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	349	38342.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	L	Pro-MCH	polymer	19	2391.9	1	UniProt (P20382) In PDB	Homo sapiens (human)
5	N	NB35	polymer	131	14225.9	1		Homo sapiens
6	R	Melanin-concentrating hormone receptor 2	polymer	331	37914.7	1	UniProt (Q969V1) Pfam (PF00001) In PDB	Homo sapiens (human)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag

R: 1 - 331 (UniProt: Q969V1)

PDB	External Database	Details
Tyr 256	Leu 256	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	128821.2
All*	Total formula weight	128821.2

*Water molecules are not included.