8WQG
cryo-EM structure of neddylated CUL2-RBX1-ELOB-ELOC-FEM1B bound with the C-degron of CCDC89 (conformation 1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | D, I | Elongin-B | polymer | 118 | 13147.8 | 2 | UniProt (Q15370) Pfam (PF00240) In PDB | Homo sapiens (human) | EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2 |
| 2 | F, J | E3 ubiquitin-protein ligase RBX1, N-terminally processed | polymer | 96 | 11196.8 | 2 | UniProt (P62877) Pfam (PF12678) In PDB | Homo sapiens (human) | E3 ubiquitin-protein transferase RBX1,N-terminally processed |
| 3 | B, H | Elongin-C | polymer | 96 | 10843.4 | 2 | UniProt (Q15369) Pfam (PF03931) In PDB | Homo sapiens (human) | EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1 |
| 4 | C, E | Cullin-2 | polymer | 750 | 87114.8 | 2 | UniProt (Q13617) Pfam (PF00888) Pfam (PF10557) In PDB | Homo sapiens (human) | CUL-2 |
| 5 | N, K | NEDD8 | polymer | 76 | 8574.0 | 2 | UniProt (Q15843) Pfam (PF00240) In PDB | Homo sapiens (human) | Neddylin,Neural precursor cell expressed developmentally down-regulated protein 8,NEDD-8,Ubiquitin-like protein Nedd8 |
| 6 | A, G | Protein fem-1 homolog B | polymer | 627 | 70355.1 | 2 | UniProt (Q9UK73) Pfam (PF12796) In PDB | Homo sapiens (human) | FEM1b,FEM1-beta,Fem-1-like death receptor-binding protein alpha,Fem-1-like in apoptotic pathway protein alpha,F1A-alpha |
| 7 | F, J | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) |
Sequence modifications
F, J: 16 - 108 (UniProt: P62877)
C, E: 2 - 745 (UniProt: Q13617)
| PDB | External Database | Details |
|---|---|---|
| Ser 13 | - | expression tag |
| His 14 | - | expression tag |
| Met 15 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -19 | - | expression tag |
| Ala -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Trp -16 | - | expression tag |
| Ser -15 | - | expression tag |
| His -14 | - | expression tag |
| Pro -13 | - | expression tag |
| Gln -12 | - | expression tag |
| Phe -11 | - | expression tag |
| Glu -10 | - | expression tag |
| Lys -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Ser -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Thr 1 | - | expression tag |
| - | Lys 117 | deletion |
| - | Leu 118 | deletion |
| - | Thr 119 | deletion |
| - | Glu 120 | deletion |
| - | Ala 121 | deletion |
| - | Asp 122 | deletion |
| - | Leu 123 | deletion |
| - | Gln 124 | deletion |
| - | Tyr 125 | deletion |
| - | Gly 126 | deletion |
| - | Tyr 127 | deletion |
| - | Gly 128 | deletion |
| - | Gly 129 | deletion |
| - | Val 130 | deletion |
| - | Asp 131 | deletion |
| Gly 132 | Met 132 | conflict |
| Gly 133 | Asn 133 | conflict |
| Gly 134 | Glu 134 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 402463.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 392.5 | |
| All* | Total formula weight | 402856.1 |
