8WQG
cryo-EM structure of neddylated CUL2-RBX1-ELOB-ELOC-FEM1B bound with the C-degron of CCDC89 (conformation 1)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.09 |
Software | cryoSPARC,PHENIX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |