8WMI
Cryo-EM structure of DiCas7-11 mutant in complex with crRNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | R | crRNA (39-MER) | polymer | 39 | 12463.3 | 1 | Escherichia coli | ||
| 2 | A | CRISPR-associated RAMP family protein | polymer | 1601 | 183845.0 | 1 | UniProt (A0A401FT36) Pfam (PF03787) In PDB | Desulfonema ishimotonii | |
| 3 | A | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
A: 1 - 1601 (UniProt: A0A401FT36)
| PDB | External Database | Details |
|---|---|---|
| Ala 429 | Asp 429 | engineered mutation |
| Ala 654 | Asp 654 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 196308.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 196570.0 |
