8WG1
Crystal structure of GH97 glucodextranase mutant E509Q from Flavobacterium johnsoniae in complex with panose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Candidate alpha-glucosidase Glycoside hydrolase family 97 | polymer | 685 | 77769.8 | 4 | UniProt (A5FBI0) Pfam (PF14508) Pfam (PF10566) Pfam (PF14509) | Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) (Cytophaga johnsonae) | |
| 2 | E, F, G, H (E, F, G, H) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 504.4 | 4 | In PDB GlyTouCan (G75128XG) | |||
| 3 | I, M, O, T (A, B, C, D) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 4 | J, K, N, P, Q... (A, B, C, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
| 5 | L, R, S (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 6 | V, W, X, Y (A, B, C, D) | water | water | 18.0 | 432 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 21 - 704 (UniProt: A5FBI0)
| PDB | External Database | Details |
|---|---|---|
| Ser 20 | - | expression tag |
| Gln 509 | Glu 509 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 311079.2 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 2017.8 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 605.6 | |
| All* | Total formula weight | 313702.6 |
