8WG1
Crystal structure of GH97 glucodextranase mutant E509Q from Flavobacterium johnsoniae in complex with panose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-25 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 290.693, 102.708, 104.529 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.230 - 2.340 |
R-factor | 0.21057 |
Rwork | 0.208 |
R-free | 0.25445 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 2.078 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.230 | 2.380 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.210 | 1.347 |
Rmeas | 0.227 | 1.453 |
Rpim | 0.086 | 0.542 |
Number of reflections | 132638 | 89573 |
<I/σ(I)> | 11.7 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.2 | 13.8 |
CC(1/2) | 0.997 | 0.734 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% PEG20000, 200 mM sodium cacodylate buffer (pH 6.0), 200 mM magnesium acetate, 10 mM glucose |