8WFS

Cryo-EM structure of GPIb-IX Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, E (c, C, B, b)	Platelet glycoprotein Ib beta chain	polymer	192	20525.9	4	UniProt (P13224)	Homo sapiens (human)
2	C, F (E, X)	Platelet glycoprotein IX	polymer	191	20346.1	2	UniProt (P14770) Pfam (PF01462) Pfam (PF13855)	Homo sapiens (human)
3	G (a)	Platelet glycoprotein Ib alpha chain	polymer	140	15587.0	1	UniProt (P07359)	Homo sapiens (human)
4	H, I, L, M (A, D, H, I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
5	J, K (F, G)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)

Sequence modifications

c, C, B, b: 1 - 181 (UniProt: P13224)

PDB	External Database	Details
Ser 148	Cys 173	engineered mutation
Gly 182	-	expression tag
Ser 183	-	expression tag
Gly 184	-	expression tag
Asp 185	-	expression tag
Tyr 186	-	expression tag
Lys 187	-	expression tag
Asp 188	-	expression tag
Asp 189	-	expression tag
Asp 190	-	expression tag
Asp 191	-	expression tag
Lys 192	-	expression tag

E, X: 1 - 161 (UniProt: P14770)

PDB	External Database	Details
Ser 154	Cys 170	engineered mutation
Ser 162	-	expression tag
Ala 163	-	expression tag
Trp 164	-	expression tag
Ser 165	-	expression tag
His 166	-	expression tag
Pro 167	-	expression tag
Gln 168	-	expression tag
Phe 169	-	expression tag
Glu 170	-	expression tag
Lys 171	-	expression tag
Gly 172	-	expression tag
Gly 173	-	expression tag
Gly 174	-	expression tag
Ser 175	-	expression tag
Gly 176	-	expression tag
Gly 177	-	expression tag
Gly 178	-	expression tag
Ser 179	-	expression tag
Gly 180	-	expression tag
Gly 181	-	expression tag
Ser 182	-	expression tag
Ala 183	-	expression tag
Trp 184	-	expression tag
Ser 185	-	expression tag
His 186	-	expression tag
Pro 187	-	expression tag
Gln 188	-	expression tag
Phe 189	-	expression tag
Glu 190	-	expression tag
Lys 191	-	expression tag

a: 481 - 610 (UniProt: P07359)

PDB	External Database	Details
Glu 559	Ser 601	engineered mutation
Glu 609	Ser 651	engineered mutation
Gly 611	-	expression tag
Gly 612	-	expression tag
His 613	-	expression tag
His 614	-	expression tag
His 615	-	expression tag
His 616	-	expression tag
His 617	-	expression tag
His 618	-	expression tag
His 619	-	expression tag
His 620	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	138382.9
Branched	Number of molecules	6
	Total formula weight	2870.7
All*	Total formula weight	141253.6

*Water molecules are not included.