8WEI

Crystal structure of Brassica napus MIK2 ectodomain (N393D mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2	polymer	702	77498.5	1	UniProt (A0A816I5A6) Pfam (PF08263) Pfam (PF13855) Pfam (PF00560)	Brassica napus (rape)
2	B, C, E (B, C, E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
3	D (D)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	1	In PDB GlyTouCan (G32152BH)
4	F (F)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
5	G (G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
6	H, I, J (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
7	K (A)	water	water		18.0	212	Chemie (HOH)

Sequence modifications

A: 1 - 690 (UniProt: A0A816I5A6)

PDB	External Database	Details
Asp 393	Asn 393	engineered mutation
Ser 546	Thr 546	conflict
Glu 691	-	expression tag
Phe 692	-	expression tag
His 693	-	expression tag
His 694	-	expression tag
His 695	-	expression tag
His 696	-	expression tag
His 697	-	expression tag
His 698	-	expression tag
His 699	-	expression tag
His 700	-	expression tag
His 701	-	expression tag
His 702	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	77498.5
Branched	Number of molecules	6
	Total formula weight	3163.0
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	81325.1

*Water molecules are not included.