8WEI

Crystal structure of Brassica napus MIK2 ectodomain (N393D mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-04-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 31 2 1
Unit cell lengths	155.895, 155.895, 126.651
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.680 - 2.710
R-factor	0.2002
Rwork	0.199
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.660
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	49088	1468
<I/σ(I)>	10.7
Completeness [%]	100.0
Redundancy	5.8
CC(1/2)	0.980	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 34% w/v PEG 400